重庆医学2016，Vol.45Issue(7)：880-882,887,4.DOI:10.3969/j.issn.1671-8348.2016.07.005
α-胞衬蛋白siRNA对人涎腺导管细胞的影响
Effect of α-Fodrin siRNA on human salivary gland cells
摘要
Abstract
Objective To observe the effect of α‐Fodrin siRNA on human salivary gland(HSG)cells and to discuss its thera‐py on sjögren′s syndrome(SS) .Methods The vectors expressing siRNA againstα‐Fodrin of human were transfected into HSG cells of 10μg α‐fodrin siRNA1 group andα‐Fodrin siRNA2 group ,while pGFP‐V‐RS vector were transfected into the cells of empty vec‐tor group ,there was no handling in HSG cell of control group .The efficiency was observed by fluorescence microscope after trans‐fection of 24 ,48 ,72 and 96 h by lipofectamine 2000 .The expression levels of α‐Fodrin mRNA and protein of HSG were detected by real‐time(RT)‐PCR and immunohistochemistry method respectively .The expression levels of IFN‐γ and IL‐10 in supernatant of cells were detected by ELISA .Results The efficiency was highest on 48 h after transfection .The level of α‐Fodrin mRNA and pro‐tein was lower in α‐fodrin siRNA1 group and α‐fodrin siRNA2 group than control group and empty vector group on 48 h after transfection (P<0 .05) ,the level of α‐Fodrin mRNA inα‐fodrin siRNA2 group was significant higher thanα‐fodrin siRNA1 group (P<0 .05) .The levels of IL‐10 were higher in α‐fodrin siRNA1 group and α‐fodrin siRNA2 group than control group and empty vector group on 48 h after transfection (P<0 .05) ,α‐fodrin siRNA1 group and α‐fodrin siRNA2 group had no statistical signifi‐cance(P>0 .05) .The levels IFN‐γ in α‐fodrin siRNA1 group and α‐fodrin siRNA2 group were lower than of control group and empty vector group on 48 h after transfection ,but there were no significant differences in the four groups (P>0 .05) .Conclusion Theα‐fodrin siRNA1 andα‐fodrin siRNA2 can suppress the levels of α‐fodrin mRNA and protein of HSG cells ,at the same time;they can elevate the expression of IL‐10 and decrease the level of IFN‐γ.Soα‐Fodrin siRNA reduce the levels of inflammatory cyto‐kines and provide experimental basis to therapy of SS .关键词
RNA, 小分子干扰/α-胞衬蛋白/人涎腺导管细胞/白细胞介素-10/干扰素-γKey words
RNA ,small interfering/α-Fodrin protein/human salivary gland cells/interleukin-10/interferon-γ分类
医药卫生引用本文复制引用
庞春艳,王蓓,剧锦哲,王永福..α-胞衬蛋白siRNA对人涎腺导管细胞的影响[J].重庆医学,2016,45(7):880-882,887,4.基金项目
包头市医药卫生基金项目（2009S1001-34）；内蒙古自治区科技计划项目（20120403）。 （）
