重庆医学2016，Vol.45Issue(8)：1009-1011,1015,4.DOI:10.3969/j.issn.1671-8348.2016.08.001
新型组合型肝肾支持系统治疗西藏小型猪急性肾衰模型的实验研究
Experimental study of Tibet miniature pig model of acute renal failure treated by a novel hybrid liver and renal support system
摘要
Abstract
Objective To evaluate the novel hybrid liver and renal support system the safety and efficacy of the treatment of Tibet mini pig model of renal failure .Methods Five Tibet miniature pig bilateral renal artery ligation approached to construct the models of renal failure ,treatment group was treated with therapy (CRRT mode) of the novel hybrid liver and renal support system after modeling ,while the control group received no treatment .Drawning venous blood endotoxin ,biochemical tests and aerobic anae‐robic culture when in the treatment of 0 ,4 ,8 h;then we observed the changes of biochemical indicators in Tibet mini pigs before and after treatment ;we recorded machine pressure monitoring indicators every 2 hour during treatment .Results The model of renal failure of two experimental animals were successfully constructed .The general condition of treatment group was improved after treated by the novel hybrid liver and renal support system ,while the control groups continued to deteriorate .The experimental ani‐mals did not appear abnormal ECG during the experiment .Two groups of animals showed no statistics significant difference (P>0 .05) in the period of 0 h .In the remaining period ,heart rate and respiratory rate in treatment group was significantly reduced(P<0 .05) ,while oxygen saturation and mean arterial pressure were significantly increased compared to control group(P<0 .05) .There was no differences of biochemical indicators between two groups before modeling (P>0 .05);after modeling both groups biochemi‐cal indicators was significantly increased compared with baseline (P<0 .05);after treatment of the hybrid liver and renal support systems in the treatment group ,the biochemical parameters were significantly decreased compared with baseline ,the difference was statistically significant different(P<0 .05) ,while the control group did not change significantly .Blood endotoxin measured results were less than 0 .5 EU/mL ,arteriovenous end aerobic anaerobic culture showed no bacterial growth .Pathological examination showed significant swelling of the renal tubular epithelium and tubular necrosis .Conclusion The novel hybrid liver and renal sup‐port system for the treatment of Tibet mini‐pig model of renal failure is safe and effective ,and it can be further attempts for clinical trials .关键词
肾功能衰竭/组合型肝肾支持系统/西藏小型猪Key words
kidney failure/hybrid liver and renal support system/Tibet miniature pig分类
医药卫生引用本文复制引用
冯磊,何国林,熊龙辉,傅超毅,霍小林,高毅..新型组合型肝肾支持系统治疗西藏小型猪急性肾衰模型的实验研究[J].重庆医学,2016,45(8):1009-1011,1015,4.基金项目
国家高技术研究发展计划（863计划）资助项目（2012AA020505）；国家自然科学基金资助项目（81470875）；广东省自然科学基金项目（2014A030312013）；广东省级科技计划项目（2014B020227002）；南方医科大学交叉学科创新团队培养计划项目；广东省医学科研基金资助项目（C1036114）。 （）
