重庆医学2016，Vol.45Issue(9)：1163-1166,4.DOI:10.3969/j.issn.1671-8348.2016.09.004
OPN干预子宫内膜异位症腺上皮细胞中NF-κBp65表达及其对细胞侵袭性的影响
Expression of NF-κBp65 in glandular epithelial cells of endometriosis after OPN intervention and its influence on cell invasiveness
摘要
Abstract
Objective To explore the influence of OPN in eutopic glandular epithelial cells of endometriosis on the NF-κBp65 expression and its relationship with the cell invasion .Methods The eutopic primary glandular epithelial cells in 12 cases of endome-triosis were performed the primary isolation and culture .The cells were collected after 24 h OPN siRNA intervention .Western blot and RT-PCR methods were adopted to detect the expressions of OPN ,NF-κBp65 protein and its mRNA before and after interven-tion .The Transwell experiment was used to detect the change of cell invasiveness before and after intervention .Results The ex-pression of OPN protein and mRNA after interfering primary glandular epithelial cells by OPN siRNA was significantly decreased , and the difference was statistically significant (t1 =7 .92 ,t2 =9 .87 ,P<0 .05) .the expression of NF-κB p65 protein and mRNA after OPN siRNA interfering primary glandular epithelial cells was obviously weakened ,the difference was statistical significant (t=2 .38 ,P<0 .05) .the invasiveness of primary glandular epithelial cells after OPN siRNA intervention was significantly decreased ,the difference was statistically significant(t=2 .38 ,P<0 .05) .The expression of OPN and NF-κBp65 had a significantly positive corre-lation in eutopic endometrial glandular epithelial cells (r=0 .87) .Conclusion The expression of OPN and NF-κBp65 is significantly decreased after OPN siRNA interfering eutopic endometrial glandular epithelial cells ,therefore OPN most likely lead to the occur-rence and development of endometriosis via the NF-κB pathway .关键词
子宫内膜异位症/骨桥蛋白质/核因子-κBp65Key words
endometriosis/osteopontin/NF-κBp65分类
医药卫生引用本文复制引用
白治苗,卢玉凤,杨眉,李望舒,哈春芳..OPN干预子宫内膜异位症腺上皮细胞中NF-κBp65表达及其对细胞侵袭性的影响[J].重庆医学,2016,45(9):1163-1166,4.基金项目
国家自然科学基金资助项目（81160078）。 （）
