重庆医学2016，Vol.45Issue(9)：1223-1227,5.DOI:10.3969/j.issn.1671-8348.2016.09.022
乳腺癌肿大腋窝淋巴结中BCSCs相关基因表达与肿瘤复发、转移的关系
Relation between expression of BCSCs associated genes from swelling axillary lymph nodes of breast cancer with recurrence and metastasis of tumor
余天雾 1龚建平 2郭丹 3周健1
作者信息
- 1. 重庆医科大学附属永川医院普外3科 402160
- 2. 重庆医科大学附属第二临床学院肝胆外科 400010
- 3. 重庆医科大学附属第二临床学院三腺外科 400010
- 折叠
摘要
Abstract
Objective To explore the relation between the expression of breast cancer stem cells(BCSCs) related gene CD44 , OCT4 and Twist1 in breast cancer tissues and swelling axillary lymph nodes with the recurrence and metastasis of breast cancer . Methods The genes and antigen expressions of BCSCs relative factors CD44 ,CD24 ,OCT4 and Twist1 in th breast cancer tissue and axillary lymph nodes were detected by the immunohistochemical method and RT‐PCR .Results Totally 14 cases of preopera‐tively and intraoperatively diagnosed breast cancer had axillary lymph nodes metastasis of cancer cells .The over expression of BC‐SCs relative factors CD44 ,OCT4 and Twist1 antigen positive in the breast cancer tissue and axillary lymph nodes were detected by the immunohistochemical method ,while CD24 was lowly expressed;the gene expressions of BCSCs relative factors CD44 ,OCT4 and Twist1 in the breast cancer tissue and axillary lymph nodes were enhanced by the RT‐PCR detection (P<0 .05) ,while CD24 gene was lowly expressed(P>0 .05) .Conclusion The genes and antigen expression of BCSCs related factors CD44 ,OCT4 and Twist1 in the patients with breast cancer complicating swelling axillary lymph nodes are significantly increased ,which might indicate that the BCSCs related factors are related with easily early metastasis and recurrence of breast cancer cells .关键词
乳腺肿瘤/干细胞/CD44/Twist1/OCT4Key words
breast neoplasms/stem cell/CD44/Twist1/OCT4分类
医药卫生引用本文复制引用
余天雾,龚建平,郭丹,周健..乳腺癌肿大腋窝淋巴结中BCSCs相关基因表达与肿瘤复发、转移的关系[J].重庆医学,2016,45(9):1223-1227,5.
