大麦与谷类科学2016，Vol.33Issue(1)：1-7,7.
西藏青稞品种藏青13高氮处理的SSH 文库构建及分析
Construction and Analysis of the SSH Libraries of the Tibetan Hulless BarleyVariety Zangqin 13 Treated with High Nitrogen
摘要
Abstract
To analyze the genes responsive to high -nitrogen treatment in Tibetan hulless barley,suppression subtractive hybridization (SSH)was utilized to construct the differential expression cDNA libraries using the hulless barley variety Zangqin 13.From the two constructed cDNA libraries,281 positive clones were randomly selected and subjected to sequencing;consequently,219 qualified ESTs containing 212 unigenes were obtained.The similarity analysis of expressed sequence tags (ESTs)of these unigenes was performed with Blastx and Blastn against the non -redundance database in GenBank.The results showed that the 212 sequences were homologous with some genes or proteins in GenBank,which are responsible for biological process,cellular components,and molecular functions according to the analysis of Blast2Go.In the analysis of KEGG,117 ESTs of the 212 sequences were mapped to 17 metabolic path-ways.Specifically,these unigenes encode enzymes that are involved in the structure and function of some metabolic synthesis path-ways;as well as in signal transduction,transcriptional regulation,and photosynthesis.This suggests that these genes may contribute to the tolerance of Tibetan hulless barley to high nitrogen treatment.关键词
青稞/高氮处理/SSH/ESTKey words
Tibetan hulless barley/High nitrogen treatment/Suppression subtractive hybridization (SSH)/Expressed sequence tags (ESTs )分类
农业科技引用本文复制引用
徐齐君,王玉林,原红军,曾兴权,尼玛扎西..西藏青稞品种藏青13高氮处理的SSH 文库构建及分析[J].大麦与谷类科学,2016,33(1):1-7,7.基金项目
国家科技支撑计划（2012BAD03B01）；西藏财政专项（2015CZZX001）。 （）
