福建农林大学学报（自然科学版）2016，Vol.45Issue(2)：162-168,7.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2016.02.007
炭疽病病原菌侵染对茶树叶片生理特性的影响
Changes in physiological properties of tea leaf infected by Colletotrichum kahawae
摘要
Abstract
To investigate pathogenicity of Colletotrichum sp.on tea leaves, a virulent strain ZRG of Colletotrichum kahawaewas isola-ted to infect 7 types of tea leaves that were commonly grown in Fujian. Physiological parameters, including relative conductivity, ma-londialdehyde(MDA)concentration and three types of antioxidase, i.e. superoxide dismutase(SOD), peroxidase(POD) and cata-lase( CAT) , were monitored at 0, 24, 48, 72 hours after infection. Subsequently, qRT-PCR was applied to express genes that were relevant to SOD, POD and CAT. Results showed that relative conductivities of infected leaves were significantly enhanced within 48h after infection. Concentrations of MDA, SOD, POD and CAT all demonstrated similar pattern, which peaked around 24th or 48th hour after inoculation and decreased afterwards. Correspondingly, qRT-PCR on SOD, POD and CAT genes detected upregulated ex-pressions of three antioxidases in infected leaves.关键词
茶树/炭疽病病原菌/生理特性/qRT-PCRKey words
tea plant/Colletotrichum sp./physiological property/qRT-PCR分类
农业科技引用本文复制引用
赖建东,魏日凤,连玲丽,刘伟,叶乃兴,杨江帆,陈丹,杨国一..炭疽病病原菌侵染对茶树叶片生理特性的影响[J].福建农林大学学报（自然科学版）,2016,45(2):162-168,7.基金项目
福建省“2011协同创新中心”项目(2015)；宁德师范学院引进人才项目(2014Y001) （2015）
