福建农林大学学报（自然科学版）2016，Vol.45Issue(2)：192-195,4.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2016.02.012
槐猪和杜洛克猪背最长肌差异表达基因的筛选与注释
Screen and annotation of differentially expressed genes in longissimus dorsi muscle of Huai pig and Duroc pig
摘要
Abstract
In order to investigate the function of differentially expressed genes and gene product on metabolism, digital gene expres-sion profiles in longissimus dorsi muscle of Huai pig ( fat) and Duroc pig ( lean) were built using RNA-seq method, and differential-ly expressed genes were screened and annotated. Gene ontology ( GO) classification analysis and significant enrichment analysis of Kyoto encyclopedia of genes and genomes ( KEGG) pathway were conducted. Results showed that 36 177 844 and 34 496 450 reads were obtained from Huai pig and Duroc pig, respectively. A total of 953 differentially expressed genes were detected between two breeds and were classified into 48 GO terms. Among the terms,13 terms were in cellular component ontology, 12 terms in molecular function ontology and 23 terms in biological process ontology. Compared to Duroc pig, Huai pig had 589 up-regulated genes and 364 down-regulated genes. All differentially expressed genes were in 6 enriched KEGG pathways, with PPAR signaling pathway relating to lipid metabolism.关键词
槐猪/杜洛克猪/背最长肌/差异表达/转录组测序Key words
Huai pig/Duroc pig/longissimus dorsi muscle/differential expression/RNA-seq分类
农业科技引用本文复制引用
杨建敏,胡宇平,林威敏,张金耀,黄守婷,叶鼎承,肖天放..槐猪和杜洛克猪背最长肌差异表达基因的筛选与注释[J].福建农林大学学报（自然科学版）,2016,45(2):192-195,4.基金项目
科技部科技基础性工作专项(2014FY120800)；福建省省属公益类科研院所专项(2014R1023-12) （2014FY120800）
