福建农林大学学报（自然科学版）2016，Vol.45Issue(2)：196-202,7.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2016.02.013
复方陈皮粉对猪胃肠道菌群多样性的影响
Effect of compound orange peel powder on pig gastrointestinal microbial diversity
摘要
Abstract
Denaturing gradient gel electrophoresis ( DGGE) and terminal-restriction fragment length polymorphism ( T-RFLP ) fin-gerprinting technique were used to study the effect of compound orange peel powder on gastrointestinal microbial diversity of swine. Sixty pigs were randomized into two groups, with the control group being fed with basal diet only while the experimental group being fed with basal diet supplemented with 0.2% compound orange peel powder for 60 days, respectively. Fresh fecal samples were col-lected after feeding, and V3 region of 16SrDNA of intestinal microorganism from the fecal samples were amplified by PCR, and the diversity of intestinal microbial was studied by DGGE. During the raising period, diarrhea frequently occurred in pigs in the control group but much less in the experimental group. Band patterns from DGGE showed that gastrointestinal microorganism was abundant in swine, and varied significantly between two groups. Furthermore, full length of 16SrDNA of intestinal microorganism was ampli-fied, with PCR products being digested by MspⅠ and HaeⅢ and followed by digested fragments being detected by T-RFLP tech-niques. Referring to recombination detection programme RDP database, 27 T-RFs fragments were found in fecal samples in both group. Most of fragments that existed in the control but not the experimental group belonged to pathogenic bacteria. To summarize, compound orange peel powder can effectively prevent pig from diarrhea and other gastrointestinal diseases clinically. This may be at-tributed to medicine that improved gastrointestinal micro-ecological environment and balanced bacteria population.关键词
复方陈皮粉/DGGE和T-RFLP指纹图谱/胃肠道微生物多样性/胃肠道菌群平衡Key words
compound orange peel powder/DGGE and T-RFLP fingerprinting/intestinal microbial diversity/intestinal bacteria population balance分类
农业科技引用本文复制引用
张文连,黄志坚,殷光文,李清禄..复方陈皮粉对猪胃肠道菌群多样性的影响[J].福建农林大学学报（自然科学版）,2016,45(2):196-202,7.基金项目
福建省自然科学基金资助项目(2013J01360) （2013J01360）
