广东牙病防治2016，Vol.24Issue(4)：204-210,7.DOI:10.12016/j.issn.2096-1456.2016.04.002
应用牙周膜干细胞—牙囊干细胞复合细胞膜片同种异体移植修复比格犬牙周组织缺损的研究
The study of the application of the periodontal membrane stem cells-dental follicle stem cells composite mem-brane allograft in the repairment of Beagle canine periodontal tissue defect
摘要
Abstract
Objective To construct the Beagle canine periodontal defect model and to observe the effect of periodontal tissue defect repairment of the periodontal membrane stem cells-dental follicle stem cells composite membrane. Methods Dog follicle stem cells ( dDFSCs) and dog periodontal ligament stem cells ( dPDLSCs) were isolated enzymatically and ex-panded in vitro. The dDFSCs, dPDLSCs and the mixed cells were used to make cell sheets separately. Then, three types of cell sheets were transplanted to dental root surfaces having three-wall periodontal defects in an allogeneic manner, and bone defects were filled with hydroxyaptite-tricalcium phosphatel ( HA-TCP) . The group only filled with HA-TCP was set as the control group. The periodontal clinical indices were evaluated at the time of 1 week and 2, 4, 6, 8 and 12 weeks after surgery. 12 weeks after surgery, the animals were sacrificed and decalcified specimens were prepared. Healing of the periodontal defects was evaluated histologically and histometrically. Results Periodontal clinical examination, histo-logical and histometrical analysis indicate that the periodontal healing of cell sheets groups were superior to the control group, and the mixed cells sheets group was superior to the other two groups ( P<0. 05) . Conclusion Allogeneic com-posite cell sheets of dDFSCs and dPDLSCs shows better effects in the repairment of Beagle periodontal tissue defect.关键词
牙囊干细胞/牙周膜干细胞/细胞膜片/同种异体移植/牙周再生Key words
Dental follicle stem cells/Periodental ligament stem cells/Cell sheet/Allogeneic transplant/Per-iodontal regeneration分类
医药卫生引用本文复制引用
李欣,金作林,吴琼,王丽颖,刘佳..应用牙周膜干细胞—牙囊干细胞复合细胞膜片同种异体移植修复比格犬牙周组织缺损的研究[J].广东牙病防治,2016,24(4):204-210,7.基金项目
国家自然科学基金(81271176) （81271176）
