解放军医药杂志2016，Vol.28Issue(3)：1-4,4.DOI:10.3969/j.issn.2095-140X.2016.03.001
4-苯基丁酸对高果糖喂养大鼠肝脏脂质沉积及氧化应激的影响
Effect of 4-phenyl Butyric Acid on Hepatic Lipid Deposition and Oxidative Stress Induced by High-fructose Feed-ing in Mice
摘要
Abstract
Objective To observe the effect of 4-phenyl butyric acid [4-PBA, an endoplasmic reticulum stress ( ERS) inhibitor] on hepatic oxidative stress induced by high-fructose feeding in mice and to investigate the effect of ERS mediation and its relationship with oxidative stress. Methods A total of 46 male Wistar rats were divided into control group (n=15), high-fructose feeding group (n=15) and 4-PBA intervention group (n=16) [0. 35 g/(kgod)4-PBA intervention was initiated in 4th week of high-fructose feeding]. After 8 weeks of feeding, all rats were sacrificed, and he-patic triglyceride ( TG) contents were detected. The expression of glucose regulated protein 78 ( GRP78 ) was detected with polymerase chain reaction ( PCR) method. Activities of superoxide dismutase ( SOD) , catalase ( CAT) and gluta-thione peroxidase (GSH-Px) and malondialdehyde (MDA) contents were detected. The expression of C/EBP homolo-gous protein ( CHOP) was detected with Western blot method. Results In high-fructose feeding group, the values of TG content and GRP78 and CHOP expressions were significantly increased compared with those in control group (P<0. 01);while the above values were significantly decreased in 4-PBA intervention group compared with those in high-fructose feeding group (P<0. 01). In high-fructose feeding group, the values of SOD, GSH-Px and CAT activities were signifi-cantly decreased, while MDA content was increased more than those in the control group (P<0. 01);in 4-PBA interven-tion group, the values of SOD, GSH-Px and CAT activities were significantly higher, while MDA content was lower than those in high-fructose feeding group (P<0. 01). Conclusion Long-term high-fructose feeding can induce hepatic ERS and oxidative stress, and 4-phenyl butyric acid can improve hepatic oxidative stress induced by high-fructose feeding.关键词
4-苯基丁酸/果糖/内质网应激/氧化应激/大鼠,WistarKey words
4-phenyl butyric acid/Fructose/Endoplasmic reticulum stress/Oxidative stress/Rats, Wistar分类
医药卫生引用本文复制引用
任路平,张璞,张雪梅,宋光耀,陈树春..4-苯基丁酸对高果糖喂养大鼠肝脏脂质沉积及氧化应激的影响[J].解放军医药杂志,2016,28(3):1-4,4.基金项目
国家自然基金资助项目(81200639) （81200639）
