解放军医药杂志2016，Vol.28Issue(3)：85-87,116,4.DOI:10.3969/j.issn.2095-140X.2016.03.020
中药蛤蚧对非酒精性脂肪肝内质网应激的影响分析
Effect of Traditional Chinese Diseases Gecko on Endoplasmic Reticulum Stress in Non-alcoholic Fatty Liver Dis-ease
摘要
Abstract
Objective To investigate the effect of traditional Chinese medicine ( TCM) Gecko on endoplasmic reticulum (ER) stress in non-alcoholic fatty liver disease (NAFLD) . Methods A total of 60 healthy male C57BL/6 mice were recruited in this study and were fed on high fat diet as the model group (n=30), while the mice were fed on common diet as the control group (n=30). The model group was divided into subgroup A, treated with TCM Gecko and subgroup B, treated with physiological saline, while the control group was divided into subgroup C, treated with TCM Gecko and subgroup D, treated with physiological saline. The ER stress of the liver was compared before and after model establishment and medication using indexes such as malondialdehyde ( MDA) , reduced glutathione ( GSH) and oxidized glutathione ( GSSG) . Results There were no significant differences in MDA, GSH and GSSG levels before model estab-lishment in the two groups (P>0. 05), and in the control group, there were no significant changes of the indexes after model establishment (P>0. 05);after model establishment in the model group, MDA and GSSG levels were increased, while GSH levels were decreased (P<0. 05). There were no obvious changes in subgroup B, C and D before and after the medication (P>0. 05). In group A, the MDA and GSSG levels were significantly decreased, while GSH level was significantly increased after the medication compared with those before the medication ( P<0. 05 ) . Conclusion TCM Gecko is effective in regulation of lipid metabolism, liver protection, anti-inflammation, anti-oxidation, enhancement of immunity and tolerance improvement of the cells to ER stress, and its application in NAFLD can protect liver tissues by starting the stress mechanisms of hepatocyte endoplasmic reticulum through lipid-lowering effect.关键词
非酒精性脂肪肝/内质网应激/中药蛤蚧/血脂调节/大鼠,近交C57BLKey words
Non-alcoholic fatty liver disease/Endoplasmic reticulum stress/Traditional Chinese medicine gecko/Regulation of blood lipid/Mice, inbred C57BL分类
医药卫生引用本文复制引用
潘磊..中药蛤蚧对非酒精性脂肪肝内质网应激的影响分析[J].解放军医药杂志,2016,28(3):85-87,116,4.基金项目
河北省中医药管理局计划项目(2015290) （2015290）
