继续医学教育2016，Vol.30Issue(3)：119-120,2.DOI:10.3969/j.issn.1004-6763.2016.03.075
浅析电镜Formvar support films制备法的改进
Analysis on the improvement of preparation method of Formvar Support Films by Electron Microscope
孙竹林1
作者信息
- 1. 首都医科大学医学实验与测试中心，北京100069
- 折叠
摘要
Abstract
Objective To study the technology improvement of Formvar film in the preparation of electron microscope laboratory, in order to improve the iflm yield, get the high quality support membrane, save the cost and increase the effective utilization rate. Methods Improve using method of diping iflm by glass slides, concentration of Formvar solution and time range with touching membrane. Results Through the practical application of biological samples for electron microscopy observation, repeatedly with a glass slide to dip Formvar solution in 0.3%concentration about 15 s, we found that the membrane has good stability in electron beam, and iflm is clean and neat, with high resolution of bearing the weight of sample, the structure is clear. Conclusion This method simpliifes the process and saves time and material, and provides reference for the daily practice.关键词
电镜实验/Formvar膜/制备法Key words
Electron microscopy experiment/Formvar membrane/Preparation method分类
医药卫生引用本文复制引用
孙竹林..浅析电镜Formvar support films制备法的改进[J].继续医学教育,2016,30(3):119-120,2.
