林业科学2016，Vol.52Issue(3)：105-111,7.DOI:10.11707/j.1001-7488.20160213
红松种子发育过程中内源激素含量的动态变化
Content Dynamics of Endogenous Hormones in Different Seed Developmental Stages of Korean Pine
摘要
Abstract
[Objective]The plant endogenous hormones,including auxin,cytokinin,gibberellin,and abscisic acid, play an important role in regulating the embryonic development of seeds. The dynamics of IAA,ZR,GAs,and ABA were studied in order to reveal the mechanism of embryonic development of Korean pine ( Pinus koraiensis) seed,as well as to provide evidence for adjusting the addition of exogenous hormones during somatic embryogenesis and/or seed development of in Korean pine seed orchard.[Method]The morphological changes of the megagametophytes of Korean pine were observed during different developmental stages,including pro-embryo,cleavage polyembryony,columnar embryo,and pre-cotyledonary embryo,and the concentration of IAA,GAs,ZR,and ABA of two Korean pine clones (057 and 059, respectively ) during the four embryonic development stages was analyzed using enzyme-linked immunosorbent assay ( ELISA) . [Results]The concentration of the endogenous hormones at the four stages of embryonic development was similar in the two clones. The content of IAA and ABA during the four stages of embryonic development of Korean pine seed was maintained at a high level. In contrast,the content of GAs remained relatively low level during the whole processes. The concentration of ZR showed a downward tendency with the development of the embryo,with the peak values being found in the pro-embryo megagametophytes:12 . 901 ng·g -1 FW in clone 057 and 13 . 622 4 ng·g -1 FW in clone 059. The IAA content increased slowly at first and then sharply at the stage of columnar,and then dramatically declined at the stage of pre-cotyledonary embryo. The IAA content in the columnar embryo was highest: 101. 923 0 and 107. 256 4 ng·g -1 FW for clone 057 and 059,respectively. The GAs content first decreased and then increased. In detail,the GAs content decreased from pro-embryo to cleavage polyembryony,with a lowest concentration of 4. 823 5 and 5. 588 8 ng·g -1 FW at the cleavage polyembryony stage for clone 057 and 059,respectively. After that,its GAs content started to increase again until the columnar embryo and all the way to pre-cotyledonary embryo stage,however,no significant difference in the GAs content was found between pro-embryo and pre-cotyledonary embryo stage. The ABA content showed a decreasing trend after the first rise. Specifically,the ABA content was lowest in pro-embryo stage and the ABA content of clone 057 and 059 was 88. 497 4 and 94. 847 3 ng·g -1 FW,respectively. Afterwards,a upward trend had been maintained until to the cleavage polyembryony stage,with the content reaching to the peak value of 133. 200 1 and 139. 983 9 ng·g -1 FW for clone 057 and 059,respectively. At the following development stages the ABA content kept decreasing and returned to the level close to pro-embryo stage. The ratio of GAs+ IAA + ZT to ABA increased at first, reached the maximum at the stage of the columnar embryo,and then decreased.[Conclusion]The content of endogenous hormones of Korean pine seeds showed a dynamic changes with the development of embryo morphology,and each hormone displayed different change pattern. In summary,IAA,ABA and ZR played an important regulatory role in embryonic development of Korean pine seeds,whereas GAs had no significant effects.关键词
红松/雌配子体/种子发育/胚胎发育/内源激素Key words
Pinus koraiensis/megagametophytes/seed development/embryonic development/endogenous hormones分类
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梁艳,沈海龙,高美玲,范震宇,朱琨,陈阳,张鹏,杨玲..红松种子发育过程中内源激素含量的动态变化[J].林业科学,2016,52(3):105-111,7.基金项目
林业公益性行业科研专项项目(201204320)和齐齐哈尔大学青年科研基金项目(2012k-M22)。 （201204320）
