南京农业大学学报2016，Vol.39Issue(2)：262-268,7.DOI:10.7685/jnau.201508001
日粮中大豆异黄酮对环境内分泌干扰物3-甲基-4-硝基酚所致公鸡肝损伤的影响
Effects of dietary soybean isoflavones on environmental endocrine disruptor 3-methyl-4-nitrophenol induced liver injury in roosters
摘要
Abstract
[Objectives] The aim of the paper is to investigate the effects of soybean isoflavones (SI)on liver injury induced by environmental endocrine disruptor 3-methyl-4-nitrophenol(PNMC) in roosters and its mechanism.[Methods] Forty-eight healthy white roosters (1 day-old)were randomly divided into 3 groups (4 replicates per group and 4 birds per replicate):basal diet group (control group),basal diet+ 10 mg· L-1 PNMC in water group (PNMC group),basal diet+ 10 mg· L-1 PNMC in water+ 100 mg· kg-1 SI in diet group (PNMC+SI group).The trial lasted 42 days and samples were collected on 21 and 42 days,respectively,and the growth performance was recorded.On the final day,livers were sampled and weighed to calculate the liver index.Activities of T-SOD,CAT and contents of MDA,GSH and H2O2 in liver tissues were assessed.In addition,morphological changes of livers were observed using HE staining.[Results] Compared with the control group,body weights in PNMC group decreased on 21 and 42 days (P＜0.05);ADG reduced during 22-42 d and 1-42 d;while ADFI and F/G significantly increased during 1-21 d,22-42 d and 1-42 d(P＜0.01);liver weight and the content of GSH were significantly lower on 42 days(P＜0.05).Compared with PNMC group,final body weight in PNMC+SI group significantly increased (P＜ 0.01);ADG was higher during 1-21 d and 22-42 d;and F/G markedly decreased during 1-21 d,22-42 d and 1-42 d(P＜0.01);meanwhile,the GSH content in PNMC+SI group was significantly enhanced on 42 days(P＜0.05),while contents of T-SOD,CAT,MDA,H2O2 of livers in PNMC group and PNMC+SI group showed no significant difference compared with those in control group(P＞0.05).Multiple hepatocyte necrosis and different degrees of nuclear condensation were observed in PNMC group,while in PNMC+SI group less serious damage was shown similar to the situation of control,alleviating the pathological changes in liver tissues.[Conclusions] These results suggested that SI could attenuate liver injury caused by PNMC,improve growth performance,and increase antioxidant ability in the liver of PNMC exposed roosters.关键词
大豆异黄酮/3-甲基-4-硝基酚/肝脏/公鸡/抗氧化能力Key words
soybean isoflavone/3-methyl-4-nitrophenol/liver/rooster/antioxidant capacity分类
生物学引用本文复制引用
徐伟风,朴元国,李延森,李春梅..日粮中大豆异黄酮对环境内分泌干扰物3-甲基-4-硝基酚所致公鸡肝损伤的影响[J].南京农业大学学报,2016,39(2):262-268,7.基金项目
江苏省自然科学基金项目(BK20131315) （BK20131315）
国家科技支撑计划项目(2012BAD39B02) （2012BAD39B02）
