农药学学报2016，Vol.18Issue(2)：165-176,12.DOI:10.16801/j.issn.1008-7303.2016.0022
姜黄素对朱砂叶螨几丁质酶基因表达的影响
Effects of curcumin on the expression of chitinase genes from the carmine spider mite,Tetranychus cinnabarinus (Boisduval)
摘要
Abstract
The mechanism of the curcumin (substance from plants) againstTetranychus cinnabarinus was elucidated. The six cDNA sequences of chitinases ofTetranychus cinnabarinus (TcCHTs) were cloned and analyzed. Quantitative RT-qPCR was used to detect the expression levels of the chitinase genes across different developmental stages ofT. cinnabarinus. Then the effect of curcumin on the gene expression ofTcCHTs was determined. The results indicated: The six CHTs cDNA sequences had different domains. These genes had predicted molecular weights of 60.80, 69.03, 104.32, 59.75, 45.63, and 49.05 kDa, respectively, and isoelectric points of 5.44, 8.65, 6.23, 6.07, 6.12 and 8.27, respectively. Quantitative RT-PCR results revealed thatCHTs expression levels in larvaes and nymphs were higher than those in eggs and adults. After the nymph mites were exposed to curcumin for 24 h, the mRNA expression levels ofTcCHTs significantly decreased. It is noteworthy thatTcCHIT1andTcCHT5 were more sensitive than others. The results indicated that one of the acaricidal mechanisms of curcumin might be inhibiting the activity of chitinase. By affecting the degradation of chitin, the epidermal degeneration was inhibited, which will lead to the damage of normal growth and development of the mites integument.关键词
姜黄素/朱砂叶螨/几丁质酶基因/基因表达Key words
curcumin/Tetranychus cinnabarinus/chitinase genes/expression分类
农业科技引用本文复制引用
王丹,章冰川,丁伟,张永强,罗金香..姜黄素对朱砂叶螨几丁质酶基因表达的影响[J].农药学学报,2016,18(2):165-176,12.基金项目
国家自然科学基金项目(31272058和31572041) （31272058和31572041）
