农药学学报2016，Vol.18Issue(2)：185-193,9.DOI:10.16801/j.issn.1008-7303.2016.0024
新型杀菌剂氟醚菌酰胺对辣椒疫霉的作用机制初探
Preliminary studies on the action mechanism of the novel fungicide LH-2010A againstPhytophthora capsici
何玲 1袁会珠 1唐剑锋 2杨代斌 1闫晓静1
作者信息
- 1. 中国农业科学院植物保护研究所/农业部作物有害生物综合治理综合性重点实验室，北京 100193
- 2. 山东中农联合生物科技股份有限公司，济南 250000
- 折叠
摘要
Abstract
The action mechanism ofN-(3-chloro-5-(trifluoromethyl) pyridine-2-methyl-2, 3, 5, 6-four fluorine-4-methoxy benzamide (LH-2010A) againstPhytophthora capsici was investigated. Bioassay method was adopted to determine the toxicity of LH-2010A against mycelial growth, zoospores release and mycelial yield ofP. capsici. Morphology and ultrastructure of mycelial treated with LH-2010A were studied. Mycelial respiration rate test was also conducted to evaluate the inhibition effect to metabolic approaches, including embden-meyerhof-parnas (EMP), tricarboxylic acid cycle (TCA) and hexose monophophate (HMP). The effect of LH-2010A on the permeability of the cell membrane,protein biosynthesis and DNA biosynthesis were determined. The results demonstrated that inhibition effect toP. capsici by LH-2010A was observed at different stages in the life cycle ofP. capsici, including the mycelial growth, the zoospores release and the mycelial yield, with EC50 values of 7.14, 16.34 and 5.12 μg/mL, respectively. Morphological and ultrastructural studies showed that LH-2010A caused the excessive branching and swelling of hyphae as well as the development of a thicker and multilayer cell wall. The inhibition of LH-2010A on mycelial growth was reduced by the addition of ATP , which suggested that the action mechanism of LH-2010A was connected with impairment of the energy generation system. Specifically, the approach of TCA was inhibited by LH-2010A significantly. The conductivity of mycelia cell suspension increased in the presence of LH-2010A, which indicated that LH-2010A affected the cell membrane permeability. The relative leakage of mycelia cell suspension treated with 100 μg/mL LH-2010A for 400 minutes was 78.23%. However, LH-2010A had little effect on the biosynthesis of protein and DNA. These results suggested that LH-2010A exhibited multiple modes of actions including the impairment of the energy generation system and the influence on the cell membrane permeability directly or indirectly.关键词
氟醚菌酰胺/辣椒疫霉/抑菌活性/作用机制Key words
LH-2010A/Phytophthora capsici/antifungal activity/action mechanism分类
农业科技引用本文复制引用
何玲,袁会珠,唐剑锋,杨代斌,闫晓静..新型杀菌剂氟醚菌酰胺对辣椒疫霉的作用机制初探[J].农药学学报,2016,18(2):185-193,9.
