农药学学报2016，Vol.18Issue(2)：219-226,8.DOI:10.16801/j.issn.1008-7303.2016.0029
直接竞争酶联免疫吸附分析法测定小白菜中溴氰菊酯的残留量
Determination of deltamethrin residue in Chinese cabbage by direct competitive enzyme-linked immunosorbent assay
摘要
Abstract
The deltamethrin polyclonal antibody and the horseradish peroxidase labeled hapten was employed to establish the direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for the determination of deltamethrin residue in Chinese cabbage. After ultrasonic extraction, concentration and dissolution with 10% methanol, the samples were determined by DC-ELISA and verified by HPLC. The results showed that the optimized concentration for antibody immobilization was 2.0 mg/L, and for HRP- hapten was 2 × 105 times diluted. The optimized coating medium was pH 7.2 PB and the optimized reaction medium was pH 6.8 PB containing 0.125 mol/L NaCl. Under the optimized conditions, the linear concentration range for the deltamethrin determination method using DC-ELISA was from 0.10 mg/L to 10 mg/L. The half maximal inhibitory concentration (IC50) of deltamethrin to antibody HRP-hapten reaction was 1.68 mg/L with relative standard deviation (RSD) of 2.11% (n = 5). And the IC10 was 0.16 mg/L. The average recoveries of deltamethrin from Chinese cabbage determined by DC-ELISA were 97%, 106% and 95% withRSDs of 8.5%, 8.1% and 8.1% (n = 5) at the spiked levels of 5 mg/kg, 1 mg/kg and 0.2 mg/kg respectively. The recovery of deltamethrin from Chinese cabbage determined by HPLC was 107% at the spiked level of 0.2 mg/kg. The DC-ELISA could be used for the rapid determination of deltamethrin residue in Chinese cabbage.关键词
直接竞争酶联免疫吸附分析/高效液相色谱/小白菜/溴氰菊酯/残留Key words
direct competitive enzyme-linked immunosorbent assay (DC-ELISA)/high performance liquid chromatography/Chinese cabbage/deltamethrin/residue分类
化学化工引用本文复制引用
孙宁浩,周引怡,王翠翠,刘曙照..直接竞争酶联免疫吸附分析法测定小白菜中溴氰菊酯的残留量[J].农药学学报,2016,18(2):219-226,8.基金项目
国家自然科学基金项目(21247002)；国家高技术研究发展计划(“863”计划)项目(2009AA03Z331) （21247002）
