农药学学报2016，Vol.18Issue(2)：227-231,5.DOI:10.16801/j.issn.1008-7303.2016.0030
九种农药制剂和一种叶面肥中有机磷和氨基甲酸酯类隐性成分的快速检测
Fast identification method of organophosphorus and carbamate in 9 kinds of pesticide formulations and 1 kind of leaf fertillizer
摘要
Abstract
A simple and rapid method was developed to detect 23 kinds of organophosphorus or carbamates pesticides which were illegally added in 9 kinds of different pesticide formulations and 1 kind of leaf fertilizer. The pesticide formulations or leaf fertilizer were diluted with phosphate buffered saline solution and the supernatant was transferred into another test tube where the cholinesterase and chromogenic reagent were added orderly. This mixture was stirred and reacted for 5 min. The final detection was performed by a pesticide residue rapid measuring instrument which is used to measure the absorbance change of the mixture during 0-3 min at the wavelength of 412 nm after the substrate was added. Additionally, the limits of detection of omethoate, phorate and methyl parathion in 9 kinds of different pesticide formulations and 1 kind of leaf fertilizers were verified. It was concluded that the limits of determination were ranged from 0.03 mg/L to 4.4 mg/L. The inhibition rates are 50% (0-26%) when the application levels in 9 kinds of different pesticide formulations and 1 kind of leaf fertilizer are marginally lower than the limit of detection. And they are 50% (56%-100%) when the application levels in 9 kinds of different pesticide formulations and 1 kind of leaf fertilizer are close to or marginally higher than the limit of detection. In conclusion, the hidden compositions mentioned above which are illegally added in pesticide formulations or leaf fertilizer could be qualitatively determined by the present method.关键词
农药制剂/叶面肥/有机磷/氨基甲酸酯/隐性成分/快速检测Key words
pesticide formulation/leaf fertilizer/organophosphorus/carbamate/hidden components/fast detection分类
农业科技引用本文复制引用
苍涛,汪志威,王新全,王祥云,吴声敢,吴长兴,陈丽萍,赵学平..九种农药制剂和一种叶面肥中有机磷和氨基甲酸酯类隐性成分的快速检测[J].农药学学报,2016,18(2):227-231,5.基金项目
公益性行业(农业)科研专项经费项目(201303088)；浙江省科技计划项目(2014C0200) （农业）
