农药学学报2016，Vol.18Issue(2)：258-262,5.DOI:10.16801/j.issn.1008-7303.2016.0034
利迪链霉菌 E12发酵液及其粗提物的抑菌活性初探
Study on the antagonistic activity of fermentation broth and its crude extract produced byStreptomyces lydicus E12
摘要
Abstract
Fermentation broth ofStreptomyces lydicus E12 displayed strong antagonistic activity against some pathogenic fungi. Effect of starch and glucose on the antifungal activity of fermentation broth was first explored by the agar diffusion method. Then antifungal substance was extracted from the fermentation broth by macroporous resin column chromatagrophy and gravity thickening. The molecular weight andβ-1,3-glucanase activity of the antifungal crude extract was detected through the reaction between the polyacrylamide gel and a thin agar gel containingβ-1,3-glucan. Results showed that the inhibition ratio of the fermentation broth dropped from 89.2% to zero when starch was replaced by glucose in the media as the solar carbon source. There was only one band on the SDS-PAGE gel with a molecular weight of about 3 kDa which showed antifungal activity, and at the corresponding position on the agar gel a clear band appeared. Take together,Streptomyces lydicus E12 can produce an antifungal substance withβ-1,3-glucanase activity.关键词
利迪链霉菌/抑菌活性/β-1,3-葡聚糖酶/聚丙烯酰胺凝胶Key words
Streptomyces lydicus/antimicrobial activity/β-1,3-glucanase/SDS-PAGE分类
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陈恳..利迪链霉菌 E12发酵液及其粗提物的抑菌活性初探[J].农药学学报,2016,18(2):258-262,5.
