四川医学2016，Vol.37Issue(3)：245-247,3.DOI:10.16252/j.cnki.issn1004-0501-2016.03.003
一种新型富血小板因子血浆制剂的动物实验安全性研究
The Safety of a Novel Preparation Platelet Factor Rich Plasma in Animal
摘要
Abstract
Objective Evaluation the safety of novel preparation Platelet Factor Rich Plasma( PFRP) in animal. Methods 30 C57 mice were randomly divided into three groups;Low Dose group(LD group),High Dose group(HD group)and Control group. PFRP were prepared according to related patent,different concentration PFRP was injected into caudal vein of LD and HD mice at a volume of 200μL every two weeks for three times respectively. Mice of Control group only received the same volume in-jection of Normal Saline. Body weight,mental activity and motor function of all three groups mice were recorded every week. Mice were sacrificed at day 42 to investigate the effect of PFRP on main organs( heart,liver,spleen,lung and kidney) by organ coefficient ration and HE staining. Results At day 14,the body weight of HD group was significantly higher than that of Control group,how-ever no obvious difference of body weight was found between different groups at any other day and no abnormal mental activity or movement was found in all three groups during the process. At day 42,organ coefficient ration of heart in HD group was significant-ly higher than that in Control group,and no other obvious difference of main organ coefficient ration was found in HD group and in LD group compared to Control group. Furthermore,HE staining revealed that there was no difference in all main organs among the three groups. Conclusion Caudal vein injection of PFRP prepared according to our patent in LD group didn’t cause abnormal e-vents on mice.关键词
富血小板因子血浆/临床前/安全性Key words
PFRP/pre-clinical/safety分类
医药卫生引用本文复制引用
张向飞,丁雪琴,陈静娴,赵俊,高雪华,海泉,赵令卉,盛琴,郑小奇,马波,王宁,雷占东..一种新型富血小板因子血浆制剂的动物实验安全性研究[J].四川医学,2016,37(3):245-247,3.基金项目
2015年四川省科技支撑计划基金资助课题(编号:2015SZ0020) （编号:2015SZ0020）
