四川中医2016，Vol.34Issue(3)：51-55,5.
电针干预对荷乳腺癌C57小鼠视交叉上核输出信号分子PK2mRNA表达的影响
Effect of Electro-acupuncture on the Expression of Pk2mRNA in SCN of C57BL/6J Mice Bearing Breast Cancer
摘要
Abstract
Objective:Observing the change of mPK2in SCN of mice bearing breast cancer after electro-acupuncture for further discuss on the output signal in SCN regulating mechanism of electro-acupuncture on the spontaneous activity rhythm of animals bearing tumors.Methods:18male C57mice were randomly divided into 3groups with 6in each group.Each group received LD domestication for 10days.After that,light source was blocked so that the mice were in free movement and they received DD domestication for 10days before model establishment started.Upon successful model establishment,the spontaneous activity rhythms of mice were monitored for 7days.After that,electro-acupuncture was administered on the electro-acupuncture group.Electro-acupuncture was administered at Changqiang (GV1) and Baihui (GV20) on CT4with HANS Acupoint and Nerves Stimulator (HANS-200) using 2/15Hz and 0.5mA for 15minutes each time and one time a day.Animals were immediately sacrificed 2hours after the third time.The sample were extracted and detected by real-time PCR.Results:The PK2mRNA levels in SCN of the model group were lower than the blank group but there were no significant differences (P＞0.05) respectively.The PK2mRNA levels in SCN of electro-acupuncture group were higher than the model group which were significantly different (P＜0.05).Conclusion:The regulating mechanism of electro-acupuncture on the spontaneous activity rhythm of tumor-bearing mice is probably in connection with the regulation of the expression of output signal PK2in SCN which should be proved by further experiments.关键词
电针/荷瘤/视交叉上核/输出信号/PK2Key words
Electro-acupuncture/Bearing tumor/SCN/Output signal/PK2分类
医药卫生引用本文复制引用
李胜吾,吴佳,薛红..电针干预对荷乳腺癌C57小鼠视交叉上核输出信号分子PK2mRNA表达的影响[J].四川中医,2016,34(3):51-55,5.基金项目
国家自然科学基金面上项目(编号:30873297). （编号:30873297）
