山东医药2016，Vol.56Issue(9)：7-9,3.DOI:10.3969/j.issn.1002-266X.2016.09.003
Th22细胞在卵巢癌恶性腹水中募集的机制
Recruitment mechanism of Th22 cells in malignant ascites of ovarian cancer
摘要
Abstract
Objective To observe the distribution of Th22 cells in malignant ascites ( MA) of ovarian cancer and to investigate its recruitment mechanism in the abdominal cavity.Methods Twenty-three cases of ovarian cancer pa-tients with MA ( observation group ) and 13 healthy women ( control group) were selected.Density gradient centrifu-gation was used to collect the mononuclear cells from MA and peripheral blood.The proportion of Th17 and Th22 cells from MA and peripheral blood, and the expression of chemokines in Th22 cells were detected by flow cytometry.The levels of IL-17 and IL-22 in MA and supernatant were examined by ELISA, and the correlations between Th22 cells and Th17 cells were determined by Spearman's rank correlation coefficients.Results The proportion of Th22 and Th17 cells in MA was significantly higher than that in peripheral blood from the observation group and control group (all P<0.05).In the observation group, the proportions of Th22 cells in MA with CCR4, CCR7 and CD45RO were higher and the proportions of Th22 cells in MA with CD45RA and CD62L were lower than those in the peripheral blood ( all P<0.05) .The levels of IL-17 and IL-22 in MA were higher than those from the peripheral blood in the observation group and control group (all P<0.05).Spearman's rank correlation coefficients showed that the propor-tion of Th22 cells was positively correlated with the proportion of Th17 cells in MA (r=0.66, P<0.01), the level of IL-22 had a positive correlation with the proportion of Th22 cells and Th17 cells in MA ( r=0.55, 0.54; all P<0 .05 ) .Conclusions Th22 cells may be recruited from peripheral blood to MA of ovarian cancer by chemokines and cytokines.关键词
卵巢肿瘤/恶性腹水/辅助T细胞22/细胞分化/细胞募集Key words
ovarian neoplasms/malignant ascites/helper T cells 22/cell differentiation/cell recruitment分类
医药卫生引用本文复制引用
卢威顺,覃山羽,姜海行,谭式辉,杨显文,罗薇,卢东红..Th22细胞在卵巢癌恶性腹水中募集的机制[J].山东医药,2016,56(9):7-9,3.基金项目
国家自然科学基金资助项目(81260083 （）
31360221) （）
