山东医药2016，Vol.56Issue(9)：10-12,3.DOI:10.3969/j.issn.1002-266X.2016.09.004
Orexin-A对大鼠海马神经细胞凋亡的影响及机制
Influence of Orexin-A on apoptosis of rat hippocampal nerve cells
摘要
Abstract
Objective To invesitage the effect of Orexin-A on the apoptosis of rat hippocampal nerve cells and the mechanism of action .Methods The primary cultured hippocampal nerve cells from 36 Wistar rats were randomly divided in the empty virus group , PLCβ1 group and PLCβ4 group which were respectively transfected by empty vectors , lentiviruses with PLCβ1 and lentiviruses with PLCβ4 .After the transfection , all three groups were further divided into subgroups treated with Orexin-A respectively for 8 h.We investigated the expression of ERK 1/2 by Western blotting and apoptosis rate was measured by flow cytometry for each subgroup .Results After treatment of Orexin-A, the expression of ERK 1/2 was in-creased and the apoptosis rate was decreased significantly in the PLCβ4 group and empty virus group (all P<0.05).How-ever, these changes in the PLCβ1 group were not obvious after treatment with Orexin-A (all P>0.05).Conclusion Orex-in-A may decrease the apoptosis of rat hippocampal nerve cells , and this function may be related with OX1R/OX2R-Gq-PLCβ1 signaling pathway .关键词
Orexin-A/海马神经细胞/细胞凋亡/细胞外信号调节激酶1/2/大鼠Key words
Orexin-A/hippocampal nerve cells/apoptosis/extracellular signal-regulated kinase 1/2/rats分类
医药卫生引用本文复制引用
池恒,李国华,唐吉友..Orexin-A对大鼠海马神经细胞凋亡的影响及机制[J].山东医药,2016,56(9):10-12,3.基金项目
山东省自然科学基金资助项目(ZR2012HM068) （ZR2012HM068）
国家自然科学基金资助项目(81471345). （81471345）
