山东医药2016，Vol.56Issue(9)：13-15,3.DOI:10.3969/j.issn.1002-266X.2016.09.005
康复新在巨噬细胞炎症反应中的抗炎作用及其机制
Anti-inflammatory effect and mechanism of Kangfuxin in inflammatory response of macrophages
摘要
Abstract
Objective To observe the anti-inflammatory effects of Kangfuxin in cell inflammation of macrophages and to discuss the possible mechanism .Methods The cultivated mouse macrophages RAW 264 .7 were randomly divided into 6 groups:group A ( added with DMSO ) , group B ( added with DMSO ) , group C ( added with anti-inflammation drug BAY11-7082), group D (added with 1%Kangfuxin), group E (added with 0.1%Kangfuxin), and group F (added with 0.01%Kangfuxin).We continued to cultivate for 1 h after drug intervention, group A was treaded with DMEM, the other groups were treated with LPS (100 μg/L) to induce cell inflammation.The cells were collected after treatment of 4 h and 8 h, the mRNA expression levels of IL-6, TNF-αand prostaglandin-endoperoxide synthase ( PTGS2) were detected by qRT-PCR.Results Four hours and eight hours after modeling , compared with group A , the mRNA expression levels of IL-6, TNF-αand PTGS2 were increased in the group B (all P<0.05); compared with group B, the mRNA expression levels of IL-6, TNF-αand PTGS2 were decreased in the group C (all P<0.05).All cells were dead in the group D.Com-pared with group B , the mRNA expression of IL-6 and TNF-αwas increased and the PTGS 2 mRNA expression was de-creased in the groups E and F (all P<0.05).Compared with group C, significant difference was found in the mRNA ex-pression of IL-6 and TNF-αbetween groups E and F (all P<0.05), and no significant difference was found in the mRNA expression of PTGS2 between groups E and F (all P>0.05).The concentration and duration of Kangfuxin had no effect on the mRNA expression of IL-6, TNF-αand PTGS2.Conclusion Kangfuxin has inhibitory effect on the cell inflammatory reaction, and its mechanism may be not related with the inhibition of expression of inflammatory factors , such as IL-6 and TNF-α, but be related with the down-regulated PTGS2 expression.关键词
炎症/康复新/抗炎/巨噬细胞/细胞炎症模型Key words
inflammation/Kangfuxin/anti-inflammation/macrophages/inflammatory response model分类
基础医学引用本文复制引用
汤雁利,张玉皓,李罡,李启艳..康复新在巨噬细胞炎症反应中的抗炎作用及其机制[J].山东医药,2016,56(9):13-15,3.基金项目
国家自然科学基金资助项目(81160134) （81160134）
云南省中青年学术和技术带头人后备人才资助项目(2012HB027) （2012HB027）
云南省医学学科带头人资助项目(D-201232). （D-201232）
