山东医药2016，Vol.56Issue(9)：16-19,4.DOI:10.3969/j.issn.1002-266X.2016.09.006
强精固肾汤对热应激雄性大鼠生殖功能的影响
Effect of Qiangjing Gushen decoction on male reproductive function of heat stress rats
摘要
Abstract
Objective To observe the protective effect of Qiangjin Gushen decoction on the heat stress-induced altered male reproductive function in rats and to investigate its possible mechanism .Methods Sixty male clean degree Wistar rats were randomly divided into the control group , model group, zinc sulfate group, low-dose, medium-dose and high-dose Qiangjing Gushen decoction groups which were orally administered with normal saline , normal saline, zinc sulfate, Qiangjin Gushen decoction 1000 mg/kg, 2000 mg/kg and 4000 mg/kg respectively for 30 d followed by exposure to heat 42℃, 1 h/d for 14 days (except for the control group ).Sex ability was observed including the capture incubation period (CIP) and the capture time (CT).On the 7th and 14th of heat stress, we detected the number of sperm count and sperm deformity rate.Meanwhile , the superoxide dismutase ( SOD) and malondialdehyde ( MDA) in the serum and testicular tissues were detected.Results Compared with the model group, on the 7th and 14th of heat stress, CIP of rats was shortened, CT was increased , the number of sperm count was increased and sperm deformity rate was decreased , the activity of SOD in the serum and testicular tissues was increased and the level of MDA in the serum and testicular tissues was decreased in the medium-dose and high-dose Qiangjing Gushen decoction groups (all P<0.05).All the above indexes had no significant statistical difference between the control group and the high-dose Qiangjing Gushen decoction group (all P>0.05).Con-clusion Qiangjing Gushen decoction can improve the male reproductive function of heat stress rats , and its mechanism may be related to the reduced oxidative damage of germ cells .关键词
热应激/强精固肾汤/生殖功能/氧化损伤/大鼠Key words
heat stress/Qiangjing Gushen decoction/reproductive function/oxidative damage/rats分类
医药卫生引用本文复制引用
张祁,贺之英,董佳琦,王以东,高俊涛..强精固肾汤对热应激雄性大鼠生殖功能的影响[J].山东医药,2016,56(9):16-19,4.基金项目
国家自然科学基金资助项目(31071042) （31071042）
吉林省大学生创新创业训练计划项目(2015022). （2015022）
