山东医药2016，Vol.56Issue(10)：1-3,3.DOI:10.3969/j.issn.1002-266X.2016.10.001
芒柄花黄素对结直肠癌细胞增殖、凋亡的影响及机制探讨
Effects of formononetin on proliferation and apoptosis of colorectal cancer cells and its mechanism
摘要
Abstract
Objective To observe the effects of formononetin on the proliferation and apoptosis of colorectal cancer cells in vitro and to study the underlying mechanism.Methods The colorectal cancer cells HCT116 were randomly divid-ed into two groups:the observation group and the control group.Cells in the observation group were treated with 25μmol/L, 50 μmol/L and 100 μmol/L formononetin, and the control group was treated with the same amount of dimethyl sulfox-ide.The proliferation ( A490 ) of HCT116 cells measured by MTT assay, the apoptosis was assessed with Hoechst 33258 staining, the expression level of HOC transcription antisense RNA ( HOTAIR) was quantified by qRT-PCR, and the ex-pression level of Bax protein and Bcl-2 protein was determined by Western blotting.Results Compared with the control group, the A490 values of the HCT116 cells constantly decreased along with the increased doses and prolonged time in the observation group (all P<0.05).The numbers of apoptosis cells in the observation group treated with 25 μmol/L, 50μmol/L and 100 μmol/L formononetin after 48 h were respectively (3.20 ±0.84), (20.20 ±1.92) and (31.00 ± 2.92)/HP, which were all higher than that[(1.6 ±1.14)/HP]of the control group (all P<0.01).Compared with the control group, the expression of Bcl-2 protein and HOTAIR was significantly decreased, but the expression of Bcl-2 protein was significantly increased in the observation group (all P<0.05).Conclusion Formononetin may inhibit the growth and induce the apoptosis of colorectal cancer cells by inhibiting HOTAIR to regulate the expression of Bcl-2 and Bax protein.关键词
结直肠癌/芒柄花黄素/HOC转录反义RNA/Bax蛋白/Bcl-2蛋白/细胞增殖/细胞凋亡Key words
colorectal carcinoma/formononetin/HOC transcription antisense RNA/Bax protein/Bcl-2 protein/cell proliferation/apoptosis分类
临床医学引用本文复制引用
李通,黎欣,高枫,黄家豪,甘嘉亮..芒柄花黄素对结直肠癌细胞增殖、凋亡的影响及机制探讨[J].山东医药,2016,56(10):1-3,3.基金项目
广西自然科学基金面上项目(2013GXNSFAA019153) （2013GXNSFAA019153）
广西高校科学技术研究项目(2013ZD015). （2013ZD015）
