山东医药2016，Vol.56Issue(10)：7-9,3.DOI:10.3969/j.issn.1002-266X.2016.10.003
沉默GOLPH3基因对胃癌细胞增殖、凋亡的影响及机制探讨
Effect of silencing GOLPH3 on proliferation and apoptosis of gastric cancer cells
摘要
Abstract
Objective To observe the effects of specifically silencing Golgi phosphoprotein 3 ( GOLPH3 ) gene on proliferation and apoptosis of gastric cancer cells, and to investigate the mechanism.Methods The human gastric carcino-ma cells SGC-7901 were randomly divided into 3 groups.Cells of group A were transfected with lentiviral-mediated siRNA sequences while cells of group B were transfected with lentiviral-mediated scramble sequences and group C without transfec-tion.The cell proliferation was detected by methyl thiazol tetrazolium ( MTT) at 72 h after transfection.The apoptotic rate was detected by flow cytometry.The protein expression of GOLPH3, mammalian target of rapamycin ( mTOR) and p-mTOR was examined by Western blotting.Results The OD value of group A was lower than those of groups B and C at different time points, the apoptotic rate of A group (2.70%±0.11%) was statistically higher than that of group B (0.42%± 0.02%) and group C (0.31%±0.02%), and the relative expression of GOLPH3 and p-mTOR protein of group A was lower than that of groups B and C (all P<0.05); no significant differences in the above indexes were found between groups B and C (all P>0.05).Conclusion Silencing GOLPH3 can inhibit the cell proliferation and promote the apopto-sis of gastric cancer cells and the mechanism may be related with the down-regulated expression of p-mTOR in SGC-7901 cells.关键词
胃癌细胞/高尔基体磷蛋白3/小干扰核糖核酸/细胞增殖/细胞凋亡/哺乳动物雷帕霉素靶蛋白Key words
gastric carcinoma cells/Golgi phosphoprotein 3/small interfering RNA/cell proliferation/cell apopto-sis/mammalian target of rapamycin分类
医药卫生引用本文复制引用
谢芳,赖铭裕,农云翠,苏婷..沉默GOLPH3基因对胃癌细胞增殖、凋亡的影响及机制探讨[J].山东医药,2016,56(10):7-9,3.基金项目
广西医疗卫生适宜技术研究与开发资金资助项目(S201415-01). （S201415-01）
