山东医药2016，Vol.56Issue(10)：17-19,3.DOI:10.3969/j.issn.1002-266X.2016.10.006
胰岛素样生长因子1受体对卵巢癌细胞增殖及化疗敏感性的影响
Effect of insulin-like growth factor 1 receptor on cell proliferation and chemotherapy sensitivity of ovarian cancer
摘要
Abstract
Objective To observe the insulin-like growth factor 1 receptor ( IGF-1R) on cell proliferation and chemo-therapy sensitivity of ovarian cancer.Methods Human ovarian cancer HO8910PM cells were randomly divided into 3 groups.Group A and B were respectively transfected by IGF-1R siRNA and nonsense siRNA, but group C without transfec-tion.The real-time PCR was used to detect IGF-1R mRNA, CCK 8 was employed to detect OD value of cell proliferation, and CCK 8 was used to detect the effective inhibitory concentration ( IC50 ) on cisplatin and carboplatin.Results After transfection for 48, 72 and 96 h, the expression of IGF-1R mRNA in the group A was lower than that of groups B and C (all P<0.05);at 72 h and 96 h, the OD value of group A was lower than those of groups B and C (all P<0.05);at 48 h, IC50 value on cisplatin and carboplatin of group A was lower than those of groups B and C ( all P<0.05) .No significant difference was found between groups B and C ( all P>0.05 ) .Conclusion IGF-1R can promote the cell proliferation, and reduce the chemotherapy sensitivity of ovarian cancer cells to cisplatin and carboplatin.关键词
卵巢癌/胰岛素样生长因子1受体/细胞增殖/顺铂/卡铂/化疗敏感性Key words
ovarian carcinoma/insulin-like growth factor 1 receptor/cell proliferation/cisplatin/carboplatin/chem-otherapy sensitivity分类
医药卫生引用本文复制引用
刘恩令,李君,周玉秀,王德华,糜若然..胰岛素样生长因子1受体对卵巢癌细胞增殖及化疗敏感性的影响[J].山东医药,2016,56(10):17-19,3.基金项目
河北省医学科学研究重点课题计划项目(ZD20140384). （ZD20140384）
