山东医药2016，Vol.56Issue(10)：20-22,3.DOI:10.3969/j.issn.1002-266X.2016.10.007
细胞自噬在儿童急性B淋巴细胞白血病化疗抵抗中的作用
Role of cellular autophagy in chemotherapy resistance of children with B-cell acute lymphocytic leukemia
摘要
Abstract
Objective We take the human B-cell acute lymphocytic leukemia cells ( BLCs) treated with vincristine ( VCR) as the models to investigate the role of cellular autophagy in chemotherapy resistance of childhood B-cell acute lym-phoblastic leukemia ( B-ALL) .Methods BLCs were randomly divided into the control group, experimental group, blocker group and union group, which were respectively treated with the same volume of PBS, 1μg/mL VCR, 10 mmol/L 3-meth-yladenine (3-MA) and 1 μg/mL VCR+10 mmol/L 3-MA for 48 h.Transmission electron microscope was used to detect the autophagosome, WST-1 was employed to calculate the proliferation inhibitory rate and Western blotting was performed to detect the autophagy-related protein light chain 3β( LC3β) expression.Results The inhibition rates after 48 h treatment in the experimental group, blocker group and union group were 9.7%±3.3%, 63.4%±9.1%and 80.6%±8.3%, re-spectively (all P<0.01).The LC3βexpression in the control group, experimental group, blocker group and union group was 0.02 ±0.01, 0.31 ±0.05, 0.01 ±0.01 and 0.02 ±0.01, respectively, and the experimental group was higher than the other groups (all P<0.01), no significant difference was found among the other three groups.Conclusion Autoph-agy can be activated during chemotherapy of ALL, leading to chemotherapy resistance.关键词
急性淋巴细胞白血病/细胞自噬/自噬相关蛋白轻链3β/化学疗法/耐药/3-甲基腺嘌呤/儿童Key words
acute lymphoblastic leukemia/autophagy/autophagy-related protein light chain 3β/chemotherapy/drug-resistance/3-methyladenine/child hood分类
医药卫生引用本文复制引用
苏莉,吕壮伟,苏敏..细胞自噬在儿童急性B淋巴细胞白血病化疗抵抗中的作用[J].山东医药,2016,56(10):20-22,3.基金项目
河南省教育厅自然科学基金项目(13A310843) （13A310843）
新乡医学院重点研究领域招标课题项目(ZD2011-12). （ZD2011-12）
