山东医药2016，Vol.56Issue(11)：5-7,3.DOI:10.3969/j.issn.1002-266X.2016.11.002
芒柄花黄素对人鼻咽癌细胞株HONE1凋亡的诱导作用及其机制探讨
Induction of formononetin on apoptosis of human nasopharyngeal carcinoma cell line HONE1 and its mechanism
摘要
Abstract
Objective To observe the induction of formononetin on apoptosis of human nasopharyngeal carcinoma cell line HONE1 and to investigate its mechanism .Methods HONE1 cells were cultured with different concentrations of form-ononetin (0, 5, 10, 20, 40, 80 and 160 μmol/L) for separated time (24, 48 and 72 h).Cell viability of HONE1 was examined using an MTT assay .The apoptosis of HONE1 cells from morphological changes after being treated with form-ononetin was examined by Hoechst33258 assay.The proteins (p-Akt, Akt, BAX and Bcl-2) of HONE1 cells were tested by Western blotting after being treated with different concentrations of formononetin (0, 5, 10, 20 and 40μmol/L) for 48 h. Results Hoechst33258 fluorescence staining showed that the number of apoptosis of HONE 1 cells was increased after being treated with formononetin as compared with the cells without treatment .The p-Akt/Akt values of HONE1 cells which were treated with different concentrations of formononetin (0, 5, 10, 20 and 40 μmol/L) for 48 h were 0.311 ±0.035, 0.169 ± 0.029, 0.116 ±0.038, 0.143 ±0.012 and 0.082 ±0.015, respectively.Significant difference was found between 0μmol/L and 5, 10, 20 and 40μmol/L (all P＜0.05).The BAX/Bcl-2 values of HONE1 cells which were treated with different con-centrations of formononetin (0, 5, 10, 20 and 40μmol/L) for 48 h were 1.445 ±0.167, 1.678 ±0.257, 2.637 ±0.619, 3.292 ±0.339 and 4.285 ±0.899, respectively.Significant difference was found between 0 μmol/L and 10, 20 and 40μmol/L (all P＜0.05).Conclusion Formononetin can induce the apoptosis of human nasopharyngeal carcinoma cell line HONE1, which may be mediated by down-regulating p-Akt/Akt and up-regulating BAX/Bcl-2.关键词
芒柄花黄素/鼻咽癌/细胞凋亡/B淋巴细胞瘤2Key words
formononetin/nasopharyngeal carcinoma/apoptosis/Bcl-2分类
药学引用本文复制引用
祁承林,梁娟,李恒,杨学敏,唐安洲..芒柄花黄素对人鼻咽癌细胞株HONE1凋亡的诱导作用及其机制探讨[J].山东医药,2016,56(11):5-7,3.基金项目
广西壮族自治区教育厅区域性高发肿瘤早期防治研究教育部重点实验室自主研究项目(GKE2015-ZZ08). （GKE2015-ZZ08）
