山东医药2016，Vol.56Issue(12)：13-15,3.DOI:10.3969/j.issn.1002-266X.2016.12.004
人胎盘间充质干细胞对小鼠肺纤维化的影响及机制
Effect of placenta-derived mesenchymal stem cells on pulmonary fibrosis in mice and its mechanism
摘要
Abstract
Objective To observe the effect of placenta-derived mesenchymal stem cells on the pulmonary fibrosis in mice and to explore its mechanism.Methods Human placenta-derived mesenchymal stem cells were isolated and cultured in vitro by tissue explants adherent method.Thirty C57BL/6 mice were randomly divided into the observation group and the control group, and all mice were injected with 8.5 mg/kg to establish the model of pulmonary fibrosis.After the models were successful established, mice in the observation group received intravenous injection of placenta-derived mesenchymal stem cells 0.3 mL (cell number of 1.0 ×106), and mice in the control group were injected with the same amount of nor-mal saline, once a day for 3 days.Then, the mice were killed to collect the lung tissues to determine the content of hydroxyproline.The expression of vascular endothelial growth factor ( VEGF ) , endothelin 1 ( ET-1 ) and angiogenin 2 ( Ang-2) proteins was determined by Western blotting.Results The hydroxyproline contents in the lung tissue of observa-tion group and control group were respectively (5.76 ±0.13) μ/mL and (8.13 ±0.87) μg/mL, and significant difference was found between the two groups (P＜0.01).The expression of VEGF, ET-1 and Ang-2 in the lung tissues of the obser-vation group was respectively 52.7 ±4.7, 68.1 ±5.4 and 59.6 ±2.8, which was all lower than that of the control group (all P＜0.05).Conclusion Placenta-derived mesenchymal stem cells can inhibit the formation of pulmonary fibrosis and its mechanism may be related with the decreased expression of VEGF, ET-1 and Ang-2 protein in lung tissue.关键词
肺纤维化/胎盘间充质干细胞/博来霉素/血管内皮生长因子/内皮素1/血管生成素2/小鼠Key words
pulmonary fibrosis/placenta-derived mesenchymal stem cells/bleomycin/vascular endothelial growth factor/endothelin 1/angiogenin 2/mice分类
医药卫生引用本文复制引用
吴树才,杨永辉,李辉,郭素敏,李秀武,杜杰杰,宋利超,高莉..人胎盘间充质干细胞对小鼠肺纤维化的影响及机制[J].山东医药,2016,56(12):13-15,3.基金项目
国家自然科学基金资助项目(31101638) （31101638）
河北省省级重大医学科研课题(ZD2013060). （ZD2013060）
