色谱2016，Vol.34Issue(4)：422-428,7.DOI:10.3724/SP.J.1123.2015.11020
在线柱后衍生-高效液相色谱-荧光检测法同时测定牛肉中16种磺胺类药物残留
Simultaneous determination of 16 sulfonamide residues in beef by high performance liquid chromatography-fluorescence detection with online post-column derivatization
摘要
Abstract
In this study,we investigated the effects of the four kinds of derivatization reagents, including fluorescamine,o-phthaladehyde,fluorescein isothiocyanate isomer and 2,3-naphtha-lenedicarboxaldehyde. A high performance liquid chromatography with fluorescence detection method for the rapid determination of 16 sulfonamide residues in beef was developed by using improved QuEChERS method for sample pretreatment and fluorescamine for online post-column derivatization. The beef samples were extracted with acetonitrile containing 1%( v/v ) acetic acid,cleaned-up by QuEChERS method,injected for online post-column derivatization with flu-orescamine,and then analysed with fluorescence detection. Under the optimized conditions of QuEChERS and chromatography,the 16 sulfonamide residues showed good linearities in the range of 0. 024-2. 533 mg/L with the correlation coefficients( r)higher than 0. 992. The limits of detection of the method were 1. 6 to 8. 2 μg/kg. The average recoveries of the 16 sulfonamide residues were in the range of 66. 6%-109. 5% with the relative standard deviations from 0. 9% to 9. 9%. The method has the advantages of rapidity,simplicity,high sensitivity and better purifi-cation effect. It is suitable for the rapid determination of the sulfonamide residues in beef.关键词
QuEChERS/荧光胺/在线柱后衍生/高效液相色谱-荧光检测法/磺胺类药物/牛肉Key words
QuEChERS/fluorescamine/online post-column derivatization/high performance liquid chromatography-fluorescence detection( HPLC-FLR)/sulfonamides/beef分类
化学化工引用本文复制引用
许旭,肖远灿,耿丹丹,皮立,董琦,胡风祖..在线柱后衍生-高效液相色谱-荧光检测法同时测定牛肉中16种磺胺类药物残留[J].色谱,2016,34(4):422-428,7.基金项目
中国科学院仪器功能开发技术创新项目（ Ig201308）；中国科学院知识创新工程重要方向项目（ KSCX2-EW-J-26）；青海省科技计划项目（2015-ZL-Y23）；青海省科技平台建设项目（2012-T-Y19）．@@@@Instrument Function Development and Technology Innovation Project of Chinese Academy of Sciences（ No． Ig201308）；Knowledge Innovative Program of Chinese Academy of Sciences（ No． KSCX2-EW-J-26）；Sci-ence and Technology Plan Project of Qinghai Province（ No．2015-ZL-Y23）；Science and Technology Plat-form Construction Project of Qinghai Province（ No．2012-T-Y19）． （）
