土壤2016，Vol.48Issue(1)：88-94,7.DOI:10.13758/j.cnki.tr.2016.01.014
强还原土壤对尖孢镰刀菌的抑制及微生物区系的影响
Influences of Reductive Soil Disinfestation onFusarium oxysporum and Soil Microbiome
摘要
Abstract
Reductive soil disinfestation (RSD) is an effective and environmentally-friendly method to suppress soil-borne pathogens. Real-time PCR and denaturing gradient gel electrophoresis were performed to investigate the influences of RSD on Fusarium oxysporumand soil microflora. Results showed that in 15 days after treatment, except for the NH3treatment, soil pH in Al-RSD and Et-RSD treatments decreased by 0.54 and 1.16 compared with that in the control treatment. NH3,Al-RSD and Et-RSD significantly reduced the numbers of Fusarium oxysporumin soil by 76%, 98% and 94%, respectively. Al-RSD significantly increased the populations and diversities of bacteria. Al-RSD and Et-RSD significantly decreased the populations of fungi in the upper soil, but increased the fungal diversities. These results indicated that RSD has a better effect on the improvement of soil microflora compared with ammonia fumigation.关键词
强还原土壤灭菌/土传病害/尖孢镰刀菌/微生物区系Key words
Reductive soil disinfestation/Soil-borne disease/Fusarium oxysporum/Soil microflora分类
农业科技引用本文复制引用
刘亮亮,黄新琦,朱睿,张金波,蔡祖聪..强还原土壤对尖孢镰刀菌的抑制及微生物区系的影响[J].土壤,2016,48(1):88-94,7.基金项目
国家自然科学基金项目(41301335)、中国博士后科学基金项目(2014M551622)和江苏高校优势学科建设工程项目资助. （41301335）
