无机材料学报2016，Vol.31Issue(3)：311-316,6.DOI:10.15541/jim20150398
KH560修饰的溶胶–凝胶法二氧化硅载体用于脂肪酶的固定化
Immobilization of Lipase on KH560 Modified Silica by Sol-Gel Process
摘要
Abstract
An epoxy-functionalized silica support was prepared through 3-glycidoxypropyltrimethoxylsilane (KH560) modified silica sol in the presence of triblock copolymer F127 (F-560-S). Surface chemistry, micromorphology and pore structure of the supports were characterized by TG, FTIR, SEM and N2 adsorption-desorption, which showed that the BET surface area and pore volume of epoxy-activated supports increased with the addition of F127. The Candida Antarctica Lipase B (CALB) was immobilized on the resulting carrier by covalent link. The amounts of lipases immobilized were 10 mg/g support and 375 mg/g support on the unmodified silica (U-S) and modified silica (F-560-S), respectively. The immobilized lipases were examined as biocatalysts for transesterification of 1-phenethanol and vinyl acetate in nonaqueous medium. The effects of solvents and temperature on immobilized lipases were systematically investigated. The catalytic activity of the CALB immobilized on F-560-S was improved in various solvents, especially in polar solvents. The immobilized CALBs maintained high activity, while the con-trol experiment using free lipase gave very low ester production in the temperature range of 0–60℃. Furthermore, thermal stability of CALB immobilized on F-560-S was improved as compared to the CALB on U-S was observed. The CALB immobilized on F-560-S exhibited high operational stability in organic media which still retained 88.3%of its original activity for 12 h consecutive 7 runs.关键词
KH560/F127/二氧化硅溶胶–凝胶/酶固定化Key words
KH560/F127/silica Sol-Gel/enzyme immobilization分类
化学化工引用本文复制引用
宋聪,喻晓蔚,钱丹,孙振忠,江波..KH560修饰的溶胶–凝胶法二氧化硅载体用于脂肪酶的固定化[J].无机材料学报,2016,31(3):311-316,6.基金项目
国家"863"计划项目(2012AA022207) National High Technology Research and Development Program of China (863 Program) (2012AA022207) （2012AA022207）
