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GA合成酶GA3OX1参与拟南芥避荫反应

董康梅 朱亚萍 王梅玲 陶懿

厦门大学学报(自然科学版)2016,Vol.55Issue(2):184-191,8.
厦门大学学报(自然科学版)2016,Vol.55Issue(2):184-191,8.DOI:10.6043/j.issn.0438-0479.2016.02.007

GA合成酶GA3OX1参与拟南芥避荫反应

GA Biosynthetic Enzyme GA3OX1 Required for Shade Avoidance in Arabidopsis

董康梅 1朱亚萍 1王梅玲 1陶懿1

作者信息

  • 1. 厦门大学生命科学学院,福建 厦门 361102
  • 折叠

摘要

Abstract

Shade avoidance response is a plastic response induced in the shade-avoiding species in response to shade.It is an important adaptive response that allows plants to compete for light when it is under or near vegetative shade.In this study,we isolated a sav8 (shadeavoidance8)mutant that was defective in multiple shade avoidance responses.We characterized the phenotypes of sav8 and conducted map-based cloning to identify the mutation in it.Compared with wild type (Col-0),sav8 had shorter hypocotyls under sim-ulated shade and dark,and adult sav8 was smaller,more branchy,and with reduced apical dominance.Under simulated shade,sav8 was hypersensitive to gibberellins (GA).Through map-based cloning,we identified that sav8 contains a C-to-T mutation in a GA synthetic gene GA3OX1 (AT1g15550),which converted Gln309 to a stop codon and encoded a truncated protein.TAA1 (tryptophan aminotransferase of Arabidopsis 1 )is a key gene required for the shade avoidance responses.Our study showed that GA3OX1 was up-regulated by shade in a TAA1-independent manner.Taking together,our study demonstrated that GA3OX1 is a key gene required for shade avoidance response.

关键词

拟南芥/避荫反应/GA3OX1/赤霉素

Key words

Arabidopsis/shade avoidance response/GA3OX1/gibberellins(GA)

分类

农业科技

引用本文复制引用

董康梅,朱亚萍,王梅玲,陶懿..GA合成酶GA3OX1参与拟南芥避荫反应[J].厦门大学学报(自然科学版),2016,55(2):184-191,8.

基金项目

福建省科技计划项目(2008F3102) (2008F3102)

厦门大学学报(自然科学版)

OA北大核心CSCDCSTPCD

0438-0479

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