局解手术学杂志2016，Vol.25Issue(3)：157-161,162,6.DOI:10.11659/jjssx.12E015143
干扰素α-1b联合环氧化酶-2抑制剂对肾癌细胞及其裸鼠移植瘤的抑制作用
Inhibitory effect of interferonα-1b and cyclooxygenase-2 inhibitor on renal cancer cell and xenografts in nude mouse
摘要
Abstract
Objective To investigate the inhibitory effect on human ACHN cell line and its mice xenograft by using interferon α-1b combined with cyclooxygenase-2 inhibitor and the relevant mechanism in vitro and vivo experiment .Methods ACHN cell and the xenograft mice were devided into 4 groups(IFN-α1b,NS398,IFNα-1b+NS398 and control group).The inhibitory effects were tested by CCK8(Cell Counting Kit 8)assay after AHCN were treated for 24 h and 48 h.The expression of bcl-xl and COX-2 were detected by Western blot .The vol-ume of the xenografts of ACHN cell line and testing the expression of VEGF in xenografts were measured by immunohistochemistry assay .Re-sults Both IFNα-1b and NS398 exerted inhibitory effects on ACHN and this effects showed a rising trend with a increasing concentration of drugs.The combined group was more significant than monotherapy group (P<0.05).Western blot assay showed that IFNα-1b and NS398 downregulated the expression of bcl-xl and COX-2 in ACHN.The combined group was more significant than monotherapy group (P<0.05). The combined group has the greatest inhibitory effects on the xenografts of ACHN cell line compared with monotherapy group and control group(P<0.05).The expression of VEGF in tumor was obiviously inhibited in combined group compared with monotherapy group and con -trol group (P<0.05).Conclusion IFNα-1b combined with NS398 can inhibit the proliferation of ACHN and suppress the tumor growth .关键词
肾癌/COX-2抑制剂/IFNα-1b/ACHN细胞/裸鼠/化疗Key words
renal cell carcinoma/cyclooxygenase-2 inhibitor/interferonα-1b/ACHN cell/nude mouse/chemotherapy分类
医药卫生引用本文复制引用
夏六兵,龙洲,张腾,董兴有,李佳,吴超,刘骞,宋波,李龙坤..干扰素α-1b联合环氧化酶-2抑制剂对肾癌细胞及其裸鼠移植瘤的抑制作用[J].局解手术学杂志,2016,25(3):157-161,162,6.基金项目
国家自然科学基金面上项目 （）
