中国康复理论与实践2016，Vol.22Issue(3)：274-277,4.DOI:10.3969/j.issn.1006-9771.2016.03.007
血清磷酸化高分子量神经微丝蛋白与大鼠脊髓损伤程度的相关性
Correlation between Serum Phosphorylated High-molecular-weight Neurofilament and Severity of Spinal Cord Injury in Rats
何件根 1李建军 2杨明亮 3武亮 2杨德刚3
作者信息
- 1. 北京小汤山医院,北京市102211
- 2. 首都医科大学康复医学院,北京市100068
- 3. 中国康复研究中心北京博爱医院,北京市100068
- 折叠
摘要
Abstract
Objective To investigate the level of phosphorylated high-molecular-weight neurofilament (pNF-H) after spinal cord injury (SCI), and explore the relationship between pNF-H and severity of SCI. Methods 20 Sprague-Dawley rats were equally divided into control group (group A), mild SCI group (group B), moderate SCI group (group C) and severe SCI group (group D). The level of pNF-H in serum, BBB score and remaining area of white matter were obtained at different time points. Results The level of serum pNF-H in groups B, C and D arrived at peaks 12 hours and 3 days after SCI, and there was significant difference among them (P<0.05). Both BBB score and remaining area of white matter 14 days after SCI negatively correlated with the level of pNF-H 3 days after SCI (r=-0.987 and r=-0.978, respective-ly). Conclusion The pNF-H increases twice after SCI in rats, and may be associated with the severe of SCI, which can be considered as a biomarker.关键词
脊髓损伤/神经微丝蛋白/分子生物学评估/大鼠Key words
spinal cord injury/neurofilament/molecular biological evaluation/rats分类
临床医学引用本文复制引用
何件根,李建军,杨明亮,武亮,杨德刚..血清磷酸化高分子量神经微丝蛋白与大鼠脊髓损伤程度的相关性[J].中国康复理论与实践,2016,22(3):274-277,4.
