中国水稻科学2016，Vol.30Issue(2)：136-142,7.DOI:10.16819/j.1001-7216.2016.5133
一个水稻白化致死突变基因的精细定位和遗传研究
Genetic Analysis and Gene Mapping of an Albino Lethal Mutant in Rice
摘要
Abstract
An albino lethal mutant,temporarily termed as al14 (albino 14 ),was obtained from 60 Coγ-ray radiated mutant pool of japonica rice variety Nipponbare.The mutant showed albino phenotype from germination,and died after 3-leaf stage.Genetic analysis revealed that the mutant phenotype was controlled by a single recessive nuclear gene. The chlorophyll and carotenoid contents in al14 declined dramatically.Transmission electron microscopy examination showed there were no obvious chloroplasts except prolamellar bodies in al14 .The expression level of most genes of photosystemⅠand photosystem Ⅱdecreased dramatically,but the ribosomal genes and RNA polymerase genes in chloroplast increased notably in al14 mutant.In al14 ,the expression amount of PsbN ,a photosystem Ⅱ protein N gene,was 118.23 times as high as that in wild type.By genetic mapping with an F2 population generated by crossing the heterozygous al14 plants with indica variety Huanghuazhan,the al14 locus was mapped within 40 kb on chromosome 6 .So far there is no reported gene relative to leaf color,so it is a novel gene controlling chloroplast development.关键词
水稻/白化致死/基因定位Key words
rice/albino/gene mapping分类
生物科学引用本文复制引用
初志战,刘小林,陈远玲,刘耀光..一个水稻白化致死突变基因的精细定位和遗传研究[J].中国水稻科学,2016,30(2):136-142,7.基金项目
亚热带农业生物资源保护与利用国家重点实验室开放课题资助项目(SKL-CUSAb-2013-04) （SKL-CUSAb-2013-04）
江西省教育厅科技计划资助项目(GJJ14707) （GJJ14707）
广东省自然科学基金-博士启动项目(2015A030310485). （2015A030310485）
