中国水稻科学2016，Vol.30Issue(2)：143-151,9.DOI:10.16819/j.1001-7216.2016.5154
水稻雄性不育突变体gamyb5的鉴定与基因定位
Identification and Gene Mapping of Male Sterile Mutant gamyb5 in Rice
摘要
Abstract
The gamyb5 ,a pollen-free male sterile mutant was identified from the mutant library of 60 Co-γ-treated indica cultivar Zhonghui 8015.There was no significant difference in agronomic traits such as plant type,plant height and tiller number between gamyb5 and the wild-type.But gamyb5 exhibited slender and white anthers without mature pollen grains.Observation results of anther cross-sections exhibited that the microspore mother cells failed to form functional tetrads and microspores in gamyb5 .Moreover,tapetal cells of gamyb5 abnormally enlarged and the tapetum programmed cell death (PCD)was delayed.gamyb5 ,as the pollen acceptor,was crossed with the wild type Zhonghui 8015 and a japonica cultivar 02428,respectively.Genetic analysis of all hybridization populations indicated that gamyb5 was controlled by a single recessive nuclear gene which was mapped on the long arm of chromosome 1 .With developed SSR,Indel markers and F2 mapping population of gamyb5/02428,the gene was fine mapped to a region of 16-kb on the long arm of chromosome 1 between markers ZF-29 and ZF-31 .Sequence analysis of the two open reading frames in this region revealed that the LOC_Os01g0812000,which encodes a gibberellin-induced MYB transcription factor,had an 8 nucleotides bases deletion in the second extron probably responsible for the male sterility phenotype. Additionally,real-time fluorescent quantitative PCR analysis showed that the expression level of the important regulators UDT1 ,TDR,CYP703A3 and CYP704B2 in anther decreased significantly in gamyb5 .Together,these results suggest GAMYB plays key roles in anther meiosis and tapetum PCD.关键词
水稻/雄性不育/基因定位/gamyb5Key words
rice/male sterility/gene mapping/gamyb5分类
生物科学引用本文复制引用
杨正福,张迎信,孙廉平,张沛沛,轩丹丹,刘嶺,胡霞,李紫荷,占小登..水稻雄性不育突变体gamyb5的鉴定与基因定位[J].中国水稻科学,2016,30(2):143-151,9.基金项目
农业部农业公益性行业科技专项(20140302,20150308) （20140302,20150308）
国家转基因重大专项(2014ZX08001-002) （2014ZX08001-002）
浙江省自然科学基金资助项目(Q13C130009) （Q13C130009）
中国农业科学院创新工程超级稻育种创新团队、水稻杂种优势机理研究创新团队项目(CAAS-ASTIP-2013-CNRRI) （CAAS-ASTIP-2013-CNRRI）
国家自然科学基金资助项目(31501290). （31501290）
