中国水稻科学2016，Vol.30Issue(2)：152-160,9.DOI:10.16819/j.1001-7216.2016.5113
水稻半显性矮秆基因Si-dd1的表型分析和精细定位
Phenotypical Analysis and Fine Mapping of a Semi-dominant Dwarfism Gene Si-dd1 in Rice
摘要
Abstract
A semi-dominant dwarf rice mutant (termed as Si-dd1)was obtained from japonica rice variety Nipponbare by tissue culture mutagenesis.Morphological analysis showed that Si-dd1 (AA)and Si-dd1 (Aa)exhibited shorter plant height,decreased seed-setting rate,delayed heading,increased number of primary rachis branch and secondary rachis branch compared to the wild type (WT,Nipponbare).Physiological test results showed Si-dd1 had similar re-sponse to 24-Epibrassinosteroid to the WT,while it was insensitive to Gibberellin at 10-4μmol/L.Moreover,Western blot results also confirmed GID2 expression level in Si-dd1 differed greatly from WT.The cytological analysis showed that Si-dd1 had smaller stomas in the leaf midrib,increased mesophyll cells and stem vascular bundles compared to WT.Genetic analysis and map-based clone showed that the Si-dd1 is controlled by a single semi-dominant gene,and locates in a 244 kb region on chromosome 6.关键词
水稻/半显性/矮秆/遗传分析/图位克隆Key words
rice/semi-dominant/dwarf/genetic analysis/map-based clone分类
生物科学引用本文复制引用
崔永涛,吴立文,胡时开,任德勇,葛常伟,叶卫军,董国军,郭龙彪,胡兴明..水稻半显性矮秆基因Si-dd1的表型分析和精细定位[J].中国水稻科学,2016,30(2):152-160,9.基金项目
深圳市科技计划资助项目(JCYJ20140504111101999) （JCYJ20140504111101999）
国家自然科学基金资助项目(31271700,31461143014). （31271700,31461143014）
