中国药业2016，Vol.25Issue(4)：24-25,26,3.
锌离子对灯盏花素与溶菌酶相互作用的影响
Effect of Zinc Iron on Interaction between Breviscapine and Lysozyme
摘要
Abstract
Objective To study the effect of Zn2 + on the interaction between breviscapine and lysozyme under the simulative human physiological condition. Methods The interaction between breviscapine with lysozyme,with or without Zn2+ was investigated using ultra-violet spectroscopy and fluorescence spectroscopy. Results The results of fluorescence spectrometry showed that the endogenous fluores-cence of lysozyme had been significantly quenched by breviscapine of fluorescence quenching were static quenching with non-radiation energy transfer. The binding parameters of breviscapine and lysozyme were as follows:with or without Zn2 +,the binding constants K were 1. 24 × 106 L/mol,1. 08 × 106 L/mol,the numbers of binding site were 1. 05,1. 19,binding distances were 1. 96 nm,2. 05 nm respec-tively. And the major driving force was hydrophobic force. Conclusion The results shows that the presence of Zn2 + does not affect the quenching mechanism,but changes the binding constants,the numbers of binding site and the binding distances.关键词
锌离子/灯盏花素/溶菌酶/荧光光谱法Key words
Zn2 +/breviscapine/lysozyme/fluorescence spectrometry分类
中医学引用本文复制引用
郑茂东,颜娟,王爱萍,赵秀花,徐今宁..锌离子对灯盏花素与溶菌酶相互作用的影响[J].中国药业,2016,25(4):24-25,26,3.基金项目
河北省自然科学基金资助项目，项目编号H4014405031；河北省卫生厅资助项目，项目编号ZD20140167；河北省张家口市科技局资助项目，项目编号1421132D。 （）
