作物学报2016，Vol.42Issue(4)：469-481,13.DOI:10.3724/SP.J.1006.2016.00469
大豆中一个WRKY28-like基因的克隆与功能分析
Cloning and Functional Analysis of a WRKY28-like Gene in Soybean
摘要
Abstract
WRKY transcription factors play important roles in regulating plant growth and development, and participate in plant response to abiotic and biotic stresses. Arabidopsis WRKY28 is an essential transcription factor for plant response to necrotrophic pathogen and abiotic stress. To explore the function of the homologues gene of AtWRKY28 in soybean, we cloned GmWRKY28-like (Glyma.14G028900) and performed bioinformatics analysis. Its sub-cellular localization and tissue expression patterns were further investigated. We also analyzed the expression levels of GmWRKY28-like under ABA, PEG, and NaCl stress treatments. Our results showed that the coding DNA sequence (CDS) of GmWRKY28-like gene is 1008 bp in length, encoding 335 amino acids. GmWRKY28-like contains a conserved WRKY domain with 22 serine, one threonine and two tyrosine, without any trans-membrane domain or signal peptide. Phylogenetic analysis showed that the WRKY28 from Phaseolus vulgaris was most similar to GmWRKY28-like. GmWRKY28-like was verified to be located in the nucleus. This gene expressed at low levels in root and seed while high levels in true leaf, flower and shoot apical meristem. The 1500 bp upstream region of GmWRKY28-like contains a variety of cis-elements, such as MBS, W box, ABRE, and box-W1, which are involved in the response to biotic and abiotic stresses, and its expression was induced by ABA, PEG, and NaCl treatments. In addition, overexpressing GmWRKY28-like in Arabidopsis thaliana enhanced plant tolerance to NaCl. This study would provide reference basis to further study the functions of GmWRKY28-like in soybean tolerance to abiotic stresses.关键词
GmWRKY28/进化树分析/亚细胞定位/非生物胁迫Key words
GmWRKY28/Phylogenetic analysis/Subcellular location/Abiotic stress引用本文复制引用
王婷婷,丛亚辉,柳聚阁,王宁,帅琴,李艳,盖钧镒..大豆中一个WRKY28-like基因的克隆与功能分析[J].作物学报,2016,42(4):469-481,13.基金项目
本研究由国家高技术研究发展计划(863计划)(2013AA102602)项目,教育部长江学者和创新团队发展计划(PCSIRT13073)项目,中央高校基本科研业务费专项资金,教育部新世纪优秀人才支持计划(NCET-12-0891)和农业部大豆生物学与遗传育种创新团队和江苏省双创计划资助。This study was supported by the National High-tech R&D Program of China (2013AA102602), the Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China (PCSIRT13073), the Fundamental Research Funds for the Central Universities, Program for New Century Excellent Talents in University of Ministry of Education of China (NCET-12-0891), Program for Soybean Biology and Genetic Breeding Innovative Research Team of Ministry of Agriculture of China, and Program for High-level Innova-tive and Entrepreneurial Talents in Jiangsu Province （863计划）
