作物学报2016，Vol.42Issue(4)：492-500,9.DOI:10.3724/SP.J.1006.2016.00492
藜麦EST-SSR的开发及通用性分析
Exploration and Transferability Evaluation of EST-SSRs in Quinoa
摘要
Abstract
Quinoa draws more and more attentions from people, since quinoa seed as grain has comprehensive nutrients. But the fundamental research on quinoa just starts and remains in a moderated level. Simple sequence repeat (SSR) development in qui-noa will enhance the resource for its genetic analysis. Here, the available RNA based sequencing (RNA-Seq) and expressed se-quence tag (EST) data of quinoa deposited in the National Center for Biotechnology Information (NCBI) were engaged in EST-SSR development. Totally, 1862 non-mononucleotide EST-SSRs were identified. Among the EST-SSRs, the dinucleotide type was the most abundant (38.3%), and the hexanucleotide was the minimal (11.7%). The amount of the EST-SSR showed the declined trend along with the increase in its motif nucleotide length. Among 119 EST-SSR primers randomly chosen for validation, 66 (55.9%) primers could give clear amplification bands and 39 showed polymorphisms in four quinoa accessions. Further analy-sis showed that the polymorphisms of EST-SSRs had no significant correlation with their motif nucleotide length. In addition, t-test demonstrated that the significant difference of EST-SSR polymorphisms was not occurred between quinoa accessions and other Chenopodiaceae germplasm. These results indicating EST-SSRs developed in quinoa could be transferable in Chenopodi-aceous genus and applied in genetic relationship analysis.关键词
藜麦/EST-SSR/分子标记/遗传关系/通用性Key words
Quinoa/EST-SSR/Molecular marker/Genetic relationship/Transferability引用本文复制引用
张体付,戚维聪,顾闽峰,张晓林,李坦,赵涵..藜麦EST-SSR的开发及通用性分析[J].作物学报,2016,42(4):492-500,9.基金项目
本研究由江苏省农业科技自主创新资金项目[CX(14)2044]和美国国际发展署Securing Water for Food Award-salt Tolerant Quinoa子项目(UR4443 B)资助。This study was supported by Jiangsu Agriculture Science and Technology Innovation Fund [CX(14)2044] and the subproject of “Securing Water for Food Award-salt Tolerant Quinoa”from USAID (UR4443 B) （14）
