作物学报2016，Vol.42Issue(4)：501-512,12.DOI:10.3724/SP.J.1006.2016.00501
甘蔗Na+/H+逆转运蛋白基因的克隆与表达分析
Isolation and Characterization of a Na+/H+Antiporter Gene from Sugarcane
摘要
Abstract
Salt overly sensitive 1 (SOS1) gene, encoding a Na+/H+antiport protein, plays an important role in biological processes of plants against salt stress. Using a SOS1 mRNA sequence from Triticum aestivum (KJ563230) as the probe, the homologous ESTs of sugarcane were obtained from NCBI database. A sugarcane cDNA sequence of SOS1 gene was cloned by in silico cloning combined with RT-PCR, and named as ScSOS1 (GenBank accession number: KT003285). The bioinformatics analysis showed that ScSOS1 has a length of 1403 bp with a complete open reading frame (ORF, 107 to 1423 bp), encoding a 423 amino acid resi-dues of sugarcane SOS1 protein with an estimated molecular weight of 47.6 kD and a calculated isoelectric point (pI) of 9.12. The protein of ScSOS1 belongs to a conserved CAP-ED superfamily. Yet the ScSOS1 protein has no signal peptide and belongs to hydrophilic protein with the main function for intermediary metabolism. The mainly secondary structure element of ScSOS1 pro-tein is random coil. Real-time quantitative PCR (RT-qPCR) analysis revealed that ScSOS1 was tissue-specifically expressed in leaf sheath, bark, pulp, bud and root of sugarcane, with the highest expression in leaf sheath and the lowest in root. Besides, the expression of ScSOS1 gene could be regulated by the treatments of NaCl, PEG, ABA, SA, and MeJA, and up-regulated by the stresses of NaCl and PEG, with the highest inducible expression levels of 1.5 times and 4.0 times as high as those of control at 24 hours, respectively. This paper suggested that ScSOS1 involves in sugarcane tolerance salt and osmotic stresses. It can set up a basis for the elucidation of sugarcane salt resistance mechanism.关键词
甘蔗/SOS1基因/电子克隆/生物信息学/实时荧光定量PCRKey words
Sugarcane/SOS1 gene/in silico cloning/Bioinformatics/Real-time quantitative PCR引用本文复制引用
刘峰,苏炜华,黄珑,肖新换,黄宁,凌辉,苏亚春,张华,阙友雄..甘蔗Na+/H+逆转运蛋白基因的克隆与表达分析[J].作物学报,2016,42(4):501-512,12.基金项目
本研究由国家现代农业产业技术体系建设专项(CARS-20),国家公益性行业(农业)科研专项(201503119)和福建省高等学校新世纪优秀人才支持计划(JA14095)资助。This study was supported by the China Agriculture Research System (CARS-20), the Special Fund for Agro-Scientific Research in the Public Interest (201503119), and the Program for New Century Excellent Talents in Fujian Province University (JA14095) （CARS-20）
