作物学报2016，Vol.42Issue(4)：513-524,12.DOI:10.3724/SP.J.1006.2016.00513
人工合成甘蓝型油菜及其亲本的甲基化变异模式分析
Analysis of DNA Methylation Patterns in Resynthesized Brassica napus and Diploid Parents
摘要
Abstract
The genetic background of Brassica napus, one of the most important oil crops in China, is relatively narrow due to the short history of its polyploid origin. Resynthesized B. napus provides an optimal model for researches on plant polyploidization. In the present study, we compared the DNA methylation levels in synthesized B. napus (F1 generation) and diploid parents using high-performance liquid chromatography (HPLC) and DNA methylation-sensitive amplification polymorphism (MSAP) analysis. HPLC analysis indicated methylation rates of 8.33%and 15.88%in B. rapa and B. oleracea, respectively. While the methylation rate of two hybrids was 10.29%and 12.83%, which were in-between of the parents’ values. MSAP analysis proved the different methylation levels in F1 generation and diploids, with the lowest and highest methylation levels identified in B. rapa and B. ole-racea, respectively. Variance of the DNA methylation in F1 was 23.71%, among which 6.60%and 10.16%were inherited from A and C genome, respectively. Sequence analysis of MSAP polymorphic fragments indicated genes involved in multiple molecular functions were changed during polyploidization. Expression analysis of these genes agreed to the corresponding methylation changes. This study provides preliminary basis for understanding epigenetic variations during B. napus polyploidization.关键词
甘蓝型油菜/人工合成种/多倍化/甲基化Key words
Bassica napus/Resynthesized species/Polyploidization/DNA methylation引用本文复制引用
谢涛,戎浩,蒋金金,孔月琴,冉丽萍,吴健,王幼平..人工合成甘蓝型油菜及其亲本的甲基化变异模式分析[J].作物学报,2016,42(4):513-524,12.基金项目
本项目由国家重点基础研究计划(973计划)项目(2015CB150201),中国和江苏省博士后基金(2014M561719,2015T80591,1401078B)资助。This study was supported by the National Key Basic Research Program of China (2015CB150201), China and Jiangsu Postdoctoral Program (2014M561719,2015T80591,1401078B) （973计划）
