作物学报2016，Vol.42Issue(4)：525-531,7.DOI:10.3724/SP.J.1006.2016.00525
紫芽茶树类黄酮生物合成关键酶基因表达与总儿茶素、花青素含量相关性分析
Correlation Analysis between Total Catechins (or Anthocyanins) and Expres-sion Levels of Genes Involved in Flavonoids Biosynthesis in Tea Plant with Purple Leaf
摘要
Abstract
Flavan-3-ols (aka catechins) and Anthocyanins found in large amounts in tea plant with purple leaf are synthesized through flavonoids metobolic pathway. To investigate the metobolic flux of flavanoids biosynthetic pathway in tea plant with pur-ple leaf, we employed a tea plant with purple leaf and a tea plant with green leaf both from Mei-Tan-Tai-Cha, to examine the ex-pression profiles of related genes involved in flavonoids biosynthesis intensively and determine the concentrations of catechins and anthocynins. The correlation between total catechins (or anthocyanin) and the expression levels of related genes were ana-lyzed. The results indicated that the expression levels of related genes (PAL, CHS, CHI, F3H, DFR, ANS, ANR1, ANR2, F3’H, and F3’5’H) were up-regulated in purple leaves as compared with those in the green leaves. The purple leaf also had higher concentra-tion of anthocyanins than the green leaf, while the green leaf was richer in total catechins. In purple leaves, the expression levels of related genes (except LAR) were highly correlated with both concentrations of total catechins (r=0.84–0.99) and anthocyanins (r = 0.72–1.00). In contrast, there was only a high correlation between the expression levels of related genes (except LAR and F3’H) and the concentration of total catechines (r=0.64–0.77) in green leaves.关键词
紫芽茶树/基因表达分析/总儿茶素/花青素/相关性分析Key words
Tea plant with purple leaf/Gene expression analysis/Total catechins/Anthocyanins/Correlation analysis引用本文复制引用
周天山,王新超,余有本,肖瑶,钱文俊,肖斌,杨亚军..紫芽茶树类黄酮生物合成关键酶基因表达与总儿茶素、花青素含量相关性分析[J].作物学报,2016,42(4):525-531,7.基金项目
本研究由国家现代农业产业技术体系建设专项(CARS-23)资助。This study was supported by the China Agriculture Research System (CARS-23) （CARS-23）
