作物学报2016，Vol.42Issue(4)：532-539,8.DOI:10.3724/SP.J.1006.2016.00532
羽衣甘蓝自交不亲和与自交亲和系种子萌发期DNA甲基化的动态变化
DNA Methylation Dynamic Analysis of Self Compatible Line and Self-Incom-patible Line of Brassica oleracea var. acephala at Seed Germination Stage
摘要
Abstract
The seed of self-incompatible line often degraded. This study aims to clarify the relationship between the degradation of the seeds and methylation. In this experiment, methylation sensitive amplification polymorphism (MSAP) was used to study the status and patterns of the DNA methylation at different periods in seed germination of self-incompatible line 9# and self-compatible line 14#. The improved CTAB method is adopted to extract seed germination in different periods of DNA, and then through the MSAP analysis, statistical amplification bands, compare the differences between 9# and 14#. The results are shown as follows:DNA methylation modification throughout the whole process of seed germination of 9#, at the early stage of the germination (0 to 2 days) methylation sites continued to increase;at the later stage (2 to 8 days) demethylation increased appar-ently, and eventually the number of demethylation was 11 times more than methylation. It was proved that DNA methylation modification was an important way to regulate the gene expression during seed germination of self-incompatible line 9#. Self-incompatible line 9# and self-compatible line 14# had different DNA methylation status clearly at the respectively periods of 0 and 2 days after seed germination. The proportion of total methylation, full-methylation and semi-methylation of 9# was all higher than of 14# at the same period. As seedings continued to grow after germination, in 9#, the proportion of full-methylation increased clearly and almost that of semi-methylation did not change, while in 14#, the proportion of semi-methylation increased clearly and almost that of full-methylation did not change.关键词
羽衣甘蓝/自交不亲和性/自交亲和性/DNA甲基化Key words
Brassica oleracea var. acephala/Self-incompatibility/Self-compatibility/DNA methylation引用本文复制引用
张旸,胡中影,赵月明,李娜,解莉楠..羽衣甘蓝自交不亲和与自交亲和系种子萌发期DNA甲基化的动态变化[J].作物学报,2016,42(4):532-539,8.基金项目
本研究由中央高校基本科研业务费专项资金项目(DL09AB13)和哈尔滨市科技创新人才研究专项资金项目(青年科技创新人才)(2013RFQXJ036)资助。This study was supported by the Basic Scientific Research Expenses of the Higher Education Institutions of Central Government, China (DL09AB13) and the Subject of Innovative Talents for Science and Technology research in Harbin (Youth Talents in Science and Technology Innovation)(2013RFQXJ036) （DL09AB13）
