作物学报2016，Vol.42Issue(4)：551-560,10.DOI:10.3724/SP.J.1006.2016.00551
水稻低叶绿素含量突变对光合作用及产量的影响
Effects of the Mutant with Low Chlorophyll Content on Photosynthesis and Yield in Rice
摘要
Abstract
A chlorophyll-deficit rice mutant (YL) with ~51% chlorophyll of its wild type (WT) was 3.7%, 20.4%, and 39.1%higher in photosynthesis than WT under saturating light condition, in treatments of 0 kg N ha–1, 120 kg N ha–1, 240 kg N ha–1, respectively. In the field and pot experiments, we studied leaf Rubisco content, stomatal conductance, expression levels of aq-uaporin genes, chlorophyll fluorescence, light and electron micrographs at different levels of N application. The results showed that the decreased level of chlorophyll content in YL was compensated by a relatively higher quantum yield of PSII. The electron micrographs of chloroplasts showed that there were no differences in chloroplast development between YL and WT. The stomatal conductance was much higher in the mutant than in wild type, and expression levels of the aquaporin genes suggested a higher mesophyll conductance in YL. The higher CO2 conductance together with a higher Rubisco content in YL could be reasons for the higher photosynthetic rate. The yield of YL was similar to that of WT, but the growth duration in YL was much shorter, which could be caused by the different photosynthetic performance between YL and WT. All these results implicate that higher photo-synthetic rate does not necessitate higher chlorophyll content. Moderate chlorophyll content will benefit the leaf photosynthesis. Decreasing N investment in chlorophyll synthesis and optimizing N distribution among different photosynthetic compounds could potentially improve photosynthesis and yield. The YL material used in this study could be potentially used to improve photosyn-thetic efficiency in breeding programmes.关键词
水稻/叶绿素/光合作用/高光效/氮素Key words
Rice/Chlorophyll/Photosynthesis/High photosynthetic efficiency/Nitrogen引用本文复制引用
顾骏飞,周振翔,李志康,戴琪星,孔祥胜,王志琴,杨建昌..水稻低叶绿素含量突变对光合作用及产量的影响[J].作物学报,2016,42(4):551-560,10.基金项目
本研究由国家重点基础研究发展计划(973计划)项目(2015CB150401),国家自然科学基金项目(31501254,31371562),江苏省自然科学基金项目(BK20140480),中国博士后科学基金(2014M550312,2015T80590),江苏省高校自然科学基金项目(14KJB210007)和江苏省高校优势学科建设项目资助。This study was supported by the National Basic Research Program of China (973 Program,2015CB150401), National Natural Science Foun-dation of China (31501254,31371562), the Natural Science Foundation of Jiangsu Province (BK20140480), China Postdoctoral Science Foundation (2014M550312,2015T80590), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (14KJB210007), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) （973计划）
