北华大学学报（自然科学版）2016，Vol.17Issue(2)：191-195,5.DOI:10.11713/j.issn.1009-4822.2016.02.010
P-5m八肽对肝癌HepG2细胞中MMP-2和MMP-9表达的抑制作用
Inhibitory Effects of P-5m Octapeptide on the Expressions of MMP-2 and MMP-9 in HepG2 Cells
摘要
Abstract
Objective To explore the impact of P-5m octapeptide on the expressions of matrix metalloproteinase-2( MMP-2) and matrix metalloproteinase-9( MMP-9) in HepG2 cells,and to investigate the antitumor mechanism of P-5m octapeptide further.Methods The HepG2 cells in logarithmic growth phase were stimulated by P-5m octapeptide for 36 h at the concentrations of 10 μmol/L and 100 μmol/L, respectively.Then, the cells were treated after 36 h.The expressive variations of MMP-2 and MMP-9 mRNA were detected by Real-time PCR,and the changes of the expressions of MMP-2 and MMP-9 proteins were detected by western blot assay.Results Real-time PCR analysis showed that there were significant differences on the expressions of MMP-2 mRNA and MMP-9 mRNA compared with the control group(P<0.05),after the cells were stimulated by P-5m octapeptide at the concentrations of 10 μmol/L and 100 μmol/L for 36 h, respectively.Western blot analysis showed that P-5m octapeptide could inhibit the expressions of MMP-2 protein and MMP-9 protein in HepG2 cells compared with the control group(P<0.05)after 36 h stimulation at the concentrations of 10 μmol/L and 100μmol/L,respectively.Conclusion P-5m octapeptide can remarkably inhibit the expressions of MMP-2 and MMP-9 in HepG2 cells.关键词
P-5m八肽/肝癌HepG2细胞/基质金属蛋白酶-2/基质金属蛋白酶-9Key words
P-5m octapeptide/HepG2 cells/matrix metalloproteinase-2/matrix metalloproteinase-9分类
医药卫生引用本文复制引用
张濛川,韩笑,安丽萍,徐广宇,李宏宇,杜培革..P-5m八肽对肝癌HepG2细胞中MMP-2和MMP-9表达的抑制作用[J].北华大学学报（自然科学版）,2016,17(2):191-195,5.基金项目
国家自然科学基金资助项目(31201061) （31201061）
吉林省发改委资助项目(2013G019) （2013G019）
吉林省科技发展计划项目(20100944 （）
20110729) （）
吉林省教育厅科学技术研究项目(2015144) （2015144）
吉林市科技局杰出青年专项(2013625030). （2013625030）
