草业学报2016，Vol.25Issue(3)：43-51,9.DOI:10.11686/cyxb2015262
阿坝地区狼毒内生放线菌多样性及抗菌活性
Diversity and anti-microbial activity of endophytic actinomycetes isolated from Stell-era chamaejasme sampled in Aba,Sichuan
摘要
Abstract
In order to develop new biologically active substances in medicinal plants,an isolation and culture method was employed to investigate the taxonomic diversity and anti-microbial activities of endophytic actino-mycetes isolated from Stellera chamaejasme sampled in Aba,Sichuan.It was found that the number of iso-lates was affected by many factors,especially organic matter content which was significantly positively correla-ted with the isolate number (P <0.05,r=0.86).Fifty two isolates were obtained on Gauze No.1 growth me-dium,accounting for 30.4% of total isolations.The numbers of isolates decreased in the following order:root, stem,leaf and flower.Analysis using 16S rDNA-RFLP divided these isolated strains into 11 clusters and fur-ther phylogenic analysis classified representative strains as genera Streptomyces ,Nocardia,Kitasatospora and Kribbella,respectively,with Streptomyces being predominant.PKS (4)and NRPS (2)genes were detected in the study and most of representative strains had antibiotic activity towards the pathogenic fungal strains, with SCAUEⅢD11-1 being most potent.It is concluded that behavior of isolates of endophytic actinomycetes from S .chamaejasme is affected by many factors with resulting diversity of expression.Functional genes and antibiotic activity indicated endophytic actinomycetes from S .chamaejasme sampled in Aba had potential me-dicinal value.关键词
狼毒/内生放线菌/多样性/功能基因/抗菌活性/系统发育Key words
Stellera chamaejasme/endophytic actinomycetes/diversity/functional genes/antibiotic activity/phylogenetic analysis引用本文复制引用
廖敏,张波,范中菡,陈熊春蕊,张小平..阿坝地区狼毒内生放线菌多样性及抗菌活性[J].草业学报,2016,25(3):43-51,9.基金项目
国家863计划（2013AA102802-05）资助。 （）
